Acholeplasmataceae 113 claims

Claim · model-written summary

A phytoplasma effector SJP4 JWB was identified and interacted with ZjWRKY40.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Gene

SJP4 JWBe0

Outcome

interact with ZjWRKY40e0

Verbatim

e0

“A phytoplasma effector SJP4 JWB was identified and shown to interact with ZjWRKY40 by yeast two‐hybrid and co‐immunoprecipitation methods.”

Claim · model-written summary

Phytoplasma detection yielded no positive results.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Outcome

failed to yield positive resultse0

Not reportedDirection

Verbatim

e0

“phytoplasma detection, which failed to yield positive results.”

Claim · model-written summary

The most effective protocol combined differential centrifugation, CTAB extraction and removal of CpG-methylated host DNA and notably increased the relative abundance of phytoplasma reads compared to the other protocols.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Treatment

differential centrifugation, CTAB extraction and removal of CpG-methylated host DNAe0

Outcome

relative abundance of phytoplasma readse0

Direction

increasee0

Comparator

other protocolse0

Verbatim

e0

“The most effective approach combined differential centrifugation, CTAB extraction and removal of CpG-methylated host DNA and resulted in a notable increase in the relative abundance of phytoplasma reads compared to other protocols”

Claim · model-written summary

Rarefaction analysis showed that the entire phytoplasma genome was covered by reads in a dataset of 3 billion nucleotides generated with this protocol.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Outcome

entire phytoplasma genome was covered by readse0

Value

3 billion nucleotidese0

Verbatim

e0

“Rarefaction analysis of the dataset generated using this protocol demonstrated that the entire phytoplasma genome was covered by reads in a dataset comprising 3 billion nucleotides”

Claim · model-written summary

Hybrid assemblies combining both technologies yielded longer phytoplasma contigs than assemblies from Illumina datasets.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Outcome

longer phytoplasma contigse0

Direction

longere0

Comparator

assemblies from Illumina datasetse0

Verbatim

e0

“hybrid assemblies that combined both technologies, yielding longer phytoplasma contigs than assemblies from Illumina datasets”

Claim · model-written summary

A hybrid de novo assembled genome of the Slovenian Flavescence doree phytoplasma isolate achieved 96% reference genome coverage with high contiguity and low error rates.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Material

Slovenian FD phytoplasma isolatee0

Outcome

reference genome coveragee0

Value

96%e0

Verbatim

e0

“A hybrid de novo assembled genome of the Slovenian FD phytoplasma isolate achieved 96% reference genome coverage, with high contiguity and low error rates”

Claim · model-written summary

SAP54 primers never yielded an amplification product.

Taxon

phytoplasmaCandidatus Phytoplasmae1

Gene

SAP54e0

Outcome

an amplification product was never obtainede0

Verbatim

e0

“In this case, using primer pairs for the SAP54 gene, an amplification product was never obtained.”

e1

“the phytoplasma, found in both periwinkle plants and insects used in transmission trials, belongs to the 16SrIII-J ribosomal subgroup”

Claim · model-written summary

RFLP analysis placed phytoplasma from trial plants and insects in subgroup 16SrIII-J.

Taxon

phytoplasmaCandidatus Phytoplasmae0

Outcome

belongs to the 16SrIII-J ribosomal subgroupe0

Verbatim

e0

“Virtual restriction fragment length polymorphism (RFLP) analysis of F2nR2 fragments indicated that the phytoplasma, found in both periwinkle plants and insects used in transmission trials, belongs to the 16SrIII-J ribosomal subgroup.”

Claim · model-written summary

Candidatus Phytoplasma vignae was proposed as a new species.

Taxon

Candidatus Phytoplasma vignaeCandidatus Phytoplasma vignaee0

Outcome

a novel speciese0

Not reportedDirection

Verbatim

e0

“As such, a novel species, ‘ Candidatus Phytoplasma vignae’, is proposed.”

Claim · model-written summary

BAWM-336 detected in bitter melon is the reference strain, with infected material deposited as VPRI 44369.

Taxon

Candidatus Phytoplasma vignaeCandidatus Phytoplasma vignaee1

Material

BAWM-336e0

Interactor

Momordica charantiaMomordica charantiae0host

Outcome

reference straine0

Qualifier

VPRI 44369e0

Not reportedValue

Verbatim

e0

“Strain BAWM-336 (genome accession number JAUZLI000000000) detected in Momordica charantia (bitter melon) serves as the reference strain of this species, with infected plant material deposited in the Victorian Plant Pathology Herbarium (VPRI) as VPRI 44369.”

e1

“a novel species, ‘ Candidatus Phytoplasma vignae’, is proposed.”