Claim · model-written summary
Pro-EndoPG I expressed in E. coli had no PG activity initially but acquired some after part of the pro-sequence was degraded by V8 protease.
Chondrostereum purpureumVerbatim
e0“It was found that Pro-EndoPG I had no PG activity initially but some was acquired after the degradation of a portion of the pro-sequence with V8 protease”
e1“The pro-form (Pro-EndoPG I) of Stereum purpureum endopolygalacturonase I”
Title
Kazuo Miyairi; Shun Ogawa; Tsubasa Kimura; et al. Journal of Applied Glycoscience 2010. doi:10.5458/jag.57.151
Other claims from this paper (5)
- Intervention effect Injection of Pro-EndoPG I into apple trees induced the same silver-leaf symptoms as mature EndoPG I.
- Mechanism It is assumed that Pro-EndoPG I was activated by plant proteases in the apple trees.
- Mechanism Deletion mutants showed that 31 or 32 of the 44 residues of the pro-sequence were needed for the inactivation of Pro-EndoPG I.
- Mechanism Two Glu residues, E364 and E366, were related to the auto-inactivation of Pro-EndoPG I.
- Mechanism The authors suggest that the pro-sequence inactivates the auto-PG activity of the enzyme.