Cryopreserved embryogenic suspension cultures of Picea sitchensis, frozen by the optimised protocol, grew at rates equal to those of untreated unfrozen cultures after thawing.

Claim · model-written summary

Cryopreserved embryogenic suspension cultures of Picea sitchensis, frozen by the optimised protocol, grew at rates equal to those of untreated unfrozen cultures after thawing.

Taxon

Picea sitchensisPicea sitchensise2

Treatment

preculture in medium containing 0.4 M sorbitol and pretreatment with 5% (v/v) DMSOe3

Outcome

growth rates of cell culturese1

Direction

equal toe1

Comparator

untreated unfrozen culturese1

Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e1

“After thawing in a waterbath at 45°C the growth rates of cell cultures cryopreserved by this method were equal to growth rates of untreated unfrozen cultures”

e2

“Embryogenic suspension cultures of sitka spruce (Picea sitchensis) were successfully cryopreserved”

e3

“The best method for cyropreservation as demonstrated by regrowth tests was: 1) preculture in medium containing 0.4 M sorbitol and pretreatment with 5% (v/v) DMSO”