Abstract
Embryogenic suspension cultures of sitka spruce (Picea sitchensis) were successfully cryopreserved. The best method for cyropreservation as demonstrated by regrowth tests was: 1) preculture in medium containing 0.4 M sorbitol and pretreatment with 5% (v/v) DMSO (dimethylsulfoxide), 2) freezing the cultures at a cooling rate of 0.5°Cmin‐1 to —40°C followed by transfer to LN (liquid nitrogen). After thawing in a waterbath at 45°C the growth rates of cell cultures cryopreserved by this method were equal to growth rates of untreated unfrozen cultures. The cryopreserved cultures gave rise to mature somatic embryos which developed cotyledons, roots and shoots 3–4 months after thawing.
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Claims 2
Claim · model-written summary
Cryopreserved embryogenic suspension cultures of Picea sitchensis, frozen by the optimised protocol, grew at rates equal to those of untreated unfrozen cultures after thawing.
- Taxon
- Picea sitchensis
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Picea sitchensise2 - Treatment
- preculture in medium containing 0.4 M sorbitol and pretreatment with 5% (v/v) DMSOe3
- Outcome
- growth rates of cell culturese1
- Direction
- equal toe1
- Comparator
- untreated unfrozen culturese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“After thawing in a waterbath at 45°C the growth rates of cell cultures cryopreserved by this method were equal to growth rates of untreated unfrozen cultures”
e2“Embryogenic suspension cultures of sitka spruce (Picea sitchensis) were successfully cryopreserved”
e3“The best method for cyropreservation as demonstrated by regrowth tests was: 1) preculture in medium containing 0.4 M sorbitol and pretreatment with 5% (v/v) DMSO”
Claim · model-written summary
Cryopreserved cultures gave rise to mature somatic embryos that developed cotyledons, roots and shoots.
- Taxon
- Picea sitchensisPicea sitchensise2
- Outcome
- mature somatic embryos which developed cotyledons, roots and shootse4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Embryogenic suspension cultures of sitka spruce (Picea sitchensis) were successfully cryopreserved”
e4“The cryopreserved cultures gave rise to mature somatic embryos which developed cotyledons, roots and shoots”
The same questions in other papers
in vitro · growth · Picea sitchensis (Sitka spruce)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.