Claim · model-written summary
Ampicillin gave the highest embryo survival rate, 42.00%.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’
Eriobotrya japonicat0 - Treatment
- Ampe0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 42.00%e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“Amp is the optimal antibiotic for the highest survival rate (42.00%) of the anther derived embryos”
Title
Lihua Lin; Li Junqiang; Hua Zong; et al. The Journal of Horticultural Science and Biotechnology 2020. doi:10.1080/14620316.2020.1814876
Other claims from this paper (13)
- Method / resource A stable transformation protocol was established for anther-derived loquat embryos.
- Intervention effect Strain EHA 52 was completely insensitive to carbenicillin at every tested concentration.
- Intervention effect Ampicillin was poisonous to both embryos and strain EHA 52.
- Intervention effect Cephalothin was poisonous to both embryos and strain EHA 52.
- Intervention effect Gentamicin was poisonous to both embryos and strain EHA 52.
- Intervention effect Gentamicin had the strongest inhibitory effect on embryo survival.
- Recommendation The authors considered Carb unsuitable for further transformation work.
- Recommendation The authors considered Gt unsuitable for further transformation work.
- Recommendation The authors suggested 800 mg·L−1 Amp with 100 mg·L−1 Kan for selecting transgenic secondary embryos.
- Observation PCR indicated secondary-embryo transformation efficiency up to 22.00%.
- Observation Transformed secondary embryo cells were smaller than cells of on-transformants.
- Observation Cell division was more rapid in transformed than non-transformed secondary embryos.
- Observation Nine KanR plantlets from two anther-derived embryos had the expected PCR fragment.