Abstract
A stable transformation protocol using Agrobacterium tumefaciens strain EHA 52 harbouring the recombinant plasmid pBI121-CycD2 for anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv. ‘Dawuxing’ was established. Parameters optimised were the concentration of antibiotics and their combinations. Results showed that strain EHA 52 is completely not sensitive to carbenicillin at any concentration. On the contrary, ampicillin (Amp), cephalothin (Cef), and gentamicin (Gt) have the poisonous action to both anther derived embryos and strain EHA 52. Amp is the optimal antibiotic for the highest survival rate (42.00%) of the anther derived embryos. Gt shows the strongest inhibitory effect on the survival of anther derived embryos. Thus, both Carb and Gt were not suitable for using in the further transformation protocol. The combination of 800 mg·L−1 Amp and 100 mg·L−1 Kan is suggested to use for selection transgenic secondary embryos. Molecular analysis (PCR) confirms that transformation efficiency of the secondary embryos is ranged from up to 22.00%. Histological observation shows that the cell volume of the secondary embryos is smaller than that of on-transformants. Moreover, the cell division rate in transformed secondary embryos is much more rapid than that in non-transformants. Finally, nine KanR plantlets from two anther derived embryos had the expected amplified fragment (0.53 Kb) by PCR analysis.
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Claims 14
Claim · model-written summary
A stable transformation protocol was established for anther-derived loquat embryos.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv. ‘Dawuxing’
Eriobotrya japonicae0 - Interactor
- Agrobacterium tumefaciens strain EHA 52
Agrobacterium tumefaciense0other - Outcome
- stable transformation protocole0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A stable transformation protocol using Agrobacterium tumefaciens strain EHA 52 harbouring the recombinant plasmid pBI121-CycD2 for anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv. ‘Dawuxing’ was established.”
Claim · model-written summary
Strain EHA 52 was completely insensitive to carbenicillin at every tested concentration.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- carbenicilline0
- Interactor
- strain EHA 52e0other
- Outcome
- strain EHA 52 is completely not sensitivee0
- Direction
- not sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“strain EHA 52 is completely not sensitive to carbenicillin at any concentration”
Claim · model-written summary
Ampicillin was poisonous to both embryos and strain EHA 52.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- ampicilline0
- Interactor
- strain EHA 52e0other
- Outcome
- poisonous action to both anther derived embryos and strain EHA 52e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“ampicillin (Amp), cephalothin (Cef), and gentamicin (Gt) have the poisonous action to both anther derived embryos and strain EHA 52”
Claim · model-written summary
Cephalothin was poisonous to both embryos and strain EHA 52.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- cephalothine0
- Interactor
- strain EHA 52e0other
- Outcome
- poisonous action to both anther derived embryos and strain EHA 52e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“ampicillin (Amp), cephalothin (Cef), and gentamicin (Gt) have the poisonous action to both anther derived embryos and strain EHA 52”
Claim · model-written summary
Gentamicin was poisonous to both embryos and strain EHA 52.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- gentamicine0
- Interactor
- strain EHA 52e0other
- Outcome
- poisonous action to both anther derived embryos and strain EHA 52e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“ampicillin (Amp), cephalothin (Cef), and gentamicin (Gt) have the poisonous action to both anther derived embryos and strain EHA 52”
Claim · model-written summary
Ampicillin gave the highest embryo survival rate, 42.00%.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- Ampe0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 42.00%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“Amp is the optimal antibiotic for the highest survival rate (42.00%) of the anther derived embryos”
Claim · model-written summary
Gentamicin had the strongest inhibitory effect on embryo survival.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- Gte0
- Outcome
- survival of anther derived embryose0
- Direction
- strongest inhibitory effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“Gt shows the strongest inhibitory effect on the survival of anther derived embryos”
Claim · model-written summary
The authors considered Carb unsuitable for further transformation work.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- Carb and Gt were not suitable for using in the further transformation protocole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“both Carb and Gt were not suitable for using in the further transformation protocol”
Claim · model-written summary
The authors considered Gt unsuitable for further transformation work.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- Carb and Gt were not suitable for using in the further transformation protocole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“both Carb and Gt were not suitable for using in the further transformation protocol”
Claim · model-written summary
The authors suggested 800 mg·L−1 Amp with 100 mg·L−1 Kan for selecting transgenic secondary embryos.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Treatment
- 800 mg·L−1 Amp and 100 mg·L−1 Kane0
- Outcome
- selection transgenic secondary embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“The combination of 800 mg·L−1 Amp and 100 mg·L−1 Kan is suggested to use for selection transgenic secondary embryos”
Claim · model-written summary
PCR indicated secondary-embryo transformation efficiency up to 22.00%.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- transformation efficiency of the secondary embryose0
- Value
- 22.00%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“Molecular analysis (PCR) confirms that transformation efficiency of the secondary embryos is ranged from up to 22.00%”
Claim · model-written summary
Transformed secondary embryo cells were smaller than cells of on-transformants.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- cell volume of the secondary embryose0
- Direction
- smallere0
- Comparator
- on-transformantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“the cell volume of the secondary embryos is smaller than that of on-transformants”
Claim · model-written summary
Cell division was more rapid in transformed than non-transformed secondary embryos.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- cell division ratee0
- Direction
- more rapide0
- Comparator
- non-transformantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“the cell division rate in transformed secondary embryos is much more rapid than that in non-transformants”
Claim · model-written summary
Nine KanR plantlets from two anther-derived embryos had the expected PCR fragment.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
- Outcome
- expected amplified fragmente0
- Value
- 0.53 Kbe0
- Qualifier
- nine KanR plantlets from two anther derived embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“nine KanR plantlets from two anther derived embryos had the expected amplified fragment (0.53 Kb) by PCR analysis”