Claim · model-written summary
The authors considered Carb unsuitable for further transformation work.
- Taxon
- Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’
Eriobotrya japonicat0 - Outcome
- Carb and Gt were not suitable for using in the further transformation protocole0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
e0“both Carb and Gt were not suitable for using in the further transformation protocol”
Title
Lihua Lin; Li Junqiang; Hua Zong; et al. The Journal of Horticultural Science and Biotechnology 2020. doi:10.1080/14620316.2020.1814876
Other claims from this paper (13)
- Method / resource A stable transformation protocol was established for anther-derived loquat embryos.
- Intervention effect Strain EHA 52 was completely insensitive to carbenicillin at every tested concentration.
- Intervention effect Ampicillin was poisonous to both embryos and strain EHA 52.
- Intervention effect Cephalothin was poisonous to both embryos and strain EHA 52.
- Intervention effect Gentamicin was poisonous to both embryos and strain EHA 52.
- Intervention effect Ampicillin gave the highest embryo survival rate, 42.00%.
- Intervention effect Gentamicin had the strongest inhibitory effect on embryo survival.
- Recommendation The authors considered Gt unsuitable for further transformation work.
- Recommendation The authors suggested 800 mg·L−1 Amp with 100 mg·L−1 Kan for selecting transgenic secondary embryos.
- Observation PCR indicated secondary-embryo transformation efficiency up to 22.00%.
- Observation Transformed secondary embryo cells were smaller than cells of on-transformants.
- Observation Cell division was more rapid in transformed than non-transformed secondary embryos.
- Observation Nine KanR plantlets from two anther-derived embryos had the expected PCR fragment.