Claim · model-written summary
Barley mesophyll protoplasts contained amino acids at concentrations up to 120 millimoles per liter.
- Taxon
- barley
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Hordeum vulgaret0 - Organ / stage
- Mesophyll protoplastse0
- Outcome
- amino acidse0
- Value
- 120 millimoles per litere0
- Qualifier
- well-fertilizede0
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
t0“Barley Mesophyll Protoplasts”
e0“Mesophyll protoplasts from leaves of well-fertilized barley (Hordeum vulgare L.) plants contained amino acids at concentrations as high as 120 millimoles per liter”
Title
Amino Acid Transport across the Tonoplast of Vacuoles Isolated from Barley Mesophyll Protoplasts
Karl-Josef Dietz; Renate Jäger; Georg Kaiser; et al. PLANT PHYSIOLOGY 1990. doi:10.1104/pp.92.1.123
Other claims from this paper (12)
- Observation Glutamic acid is predominantly localized in the cytoplasm of barley mesophyll cells.
- Observation A major part of all other amino acids is contained inside the large central vacuole of barley mesophyll cells.
- Observation Alanine, leucine and glutamine are the dominant vacuolar amino acids in barley.
- Intervention effect ATP and adenylyl imidodiphosphate stimulated amino acid uptake into barley vacuoles three- to sixfold compared with no ATP.
- Intervention effect Other nucleotides did not increase the rate of amino acid uptake into barley vacuoles.
- Observation ATP-stimulated amino acid transport into barley vacuoles does not depend on transtonoplast pH or membrane potential.
- Intervention effect p-Chloromercuriphenylsulfonic acid increased amino acid transport independently of ATP.
- Intervention effect n-Ethyl maleimide increased amino acid transport independently of ATP.
- Intervention effect Neutral amino acids such as valine or leucine decreased the rate of alanine transport.
- Intervention effect Glutamine was less effective or not effective as a competitive inhibitor of alanine transport.
- Intervention effect Glycine was less effective or not effective as a competitive inhibitor of alanine transport.
- Mechanism The results indicate a uniport translocator specific for neutral or basic amino acids in barley vacuoles, controlled by metabolic effectors.