Claim · model-written summary
p-Chloromercuriphenylsulfonic acid increased amino acid transport independently of ATP.
- Taxon
- barley
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Hordeum vulgaret0 - Treatment
- p-Chloromercuriphenylsulfonic acide7
- Outcome
- transporte7
- Direction
- increasede7
- Qualifier
- independently of ATPe7
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
t0“Barley Mesophyll Protoplasts”
e7“p-Chloromercuriphenylsulfonic acid and n-ethyl maleimide increased transport independently of ATP”
Title
Amino Acid Transport across the Tonoplast of Vacuoles Isolated from Barley Mesophyll Protoplasts
Karl-Josef Dietz; Renate Jäger; Georg Kaiser; et al. PLANT PHYSIOLOGY 1990. doi:10.1104/pp.92.1.123
Other claims from this paper (12)
- Observation Barley mesophyll protoplasts contained amino acids at concentrations up to 120 millimoles per liter.
- Observation Glutamic acid is predominantly localized in the cytoplasm of barley mesophyll cells.
- Observation A major part of all other amino acids is contained inside the large central vacuole of barley mesophyll cells.
- Observation Alanine, leucine and glutamine are the dominant vacuolar amino acids in barley.
- Intervention effect ATP and adenylyl imidodiphosphate stimulated amino acid uptake into barley vacuoles three- to sixfold compared with no ATP.
- Intervention effect Other nucleotides did not increase the rate of amino acid uptake into barley vacuoles.
- Observation ATP-stimulated amino acid transport into barley vacuoles does not depend on transtonoplast pH or membrane potential.
- Intervention effect n-Ethyl maleimide increased amino acid transport independently of ATP.
- Intervention effect Neutral amino acids such as valine or leucine decreased the rate of alanine transport.
- Intervention effect Glutamine was less effective or not effective as a competitive inhibitor of alanine transport.
- Intervention effect Glycine was less effective or not effective as a competitive inhibitor of alanine transport.
- Mechanism The results indicate a uniport translocator specific for neutral or basic amino acids in barley vacuoles, controlled by metabolic effectors.