Claim · model-written summary
At 24 h imbibition, 700 mM sucrose improved protoplast dehydration tolerance relative to 500 mM sucrose/raffinose.
- Treatment
- 700 mM sucrosee0
- Outcome
- tolerance of dehydratione0
- Direction
- improvede0
- Qualifier
- 24 h of imbibitione0
- Comparator
- 500 mM sucrose/raffinosee0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValue
Verbatim
e0“A comparison of protoplast survival after isolation and dehydration in either 500 mM sucrose/raffinose or 700 mM sucrose revealed no difference in tolerance except at 24 h of imbibition, when protoplasts treated in the more concentrated solution had improved tolerance of dehydration”
Title
Changing desiccation tolerance of pea embryo protoplasts during germination
K. L. Koster Journal of Experimental Botany 2003. doi:10.1093/jxb/erg170
Other claims from this paper (8)
- Association Protoplast survival declined as water content declined.
- Observation Protoplasts from tolerant embryos at 12 and 18 h were less dehydration sensitive than those from sensitive axes at 24 and 36 h.
- Observation WC50 increased through germination and early seedling growth in intact tissue and derived protoplasts.
- Observation Before radicle emergence, protoplasts tolerated desiccation less than intact axes.
- Observation After emergence, radicle protoplasts had lower WC50 than intact radicles.
- Intervention effect Outside 24 h imbibition, the two sucrose solutions did not differ in protoplast tolerance.
- Observation Intact epicotyls generally tolerated desiccation more than radicles.
- Observation Epicotyl and radicle protoplasts did not differ in desiccation tolerance.