Claim · model-written summary
Protoplasts from tolerant embryos at 12 and 18 h were less dehydration sensitive than those from sensitive axes at 24 and 36 h.
- Organ / stage
- protoplastse0
- Outcome
- sensitivity to dehydratione0
- Direction
- lesse0
- Comparator
- axes that were sensitivee0
Not reportedTaxonMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingValueQualifier
Verbatim
e0“the sensitivity to dehydration was less when the protoplasts were isolated from embryos that were still desiccation-tolerant (12 h and 18 h of imbibition) than when protoplasts were derived from axes that were sensitive (24 h and 36 h of imbibition)”
Title
Changing desiccation tolerance of pea embryo protoplasts during germination
K. L. Koster Journal of Experimental Botany 2003. doi:10.1093/jxb/erg170
Other claims from this paper (8)
- Association Protoplast survival declined as water content declined.
- Observation WC50 increased through germination and early seedling growth in intact tissue and derived protoplasts.
- Observation Before radicle emergence, protoplasts tolerated desiccation less than intact axes.
- Observation After emergence, radicle protoplasts had lower WC50 than intact radicles.
- Intervention effect At 24 h imbibition, 700 mM sucrose improved protoplast dehydration tolerance relative to 500 mM sucrose/raffinose.
- Intervention effect Outside 24 h imbibition, the two sucrose solutions did not differ in protoplast tolerance.
- Observation Intact epicotyls generally tolerated desiccation more than radicles.
- Observation Epicotyl and radicle protoplasts did not differ in desiccation tolerance.