Claim · model-written summary
Outside 24 h imbibition, the two sucrose solutions did not differ in protoplast tolerance.
- Treatment
- 700 mM sucrosee0
- Outcome
- tolerancee0
- Direction
- no differencee0
- Comparator
- 500 mM sucrose/raffinosee0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Verbatim
e0“A comparison of protoplast survival after isolation and dehydration in either 500 mM sucrose/raffinose or 700 mM sucrose revealed no difference in tolerance except at 24 h of imbibition”
Title
Changing desiccation tolerance of pea embryo protoplasts during germination
K. L. Koster Journal of Experimental Botany 2003. doi:10.1093/jxb/erg170
Other claims from this paper (8)
- Association Protoplast survival declined as water content declined.
- Observation Protoplasts from tolerant embryos at 12 and 18 h were less dehydration sensitive than those from sensitive axes at 24 and 36 h.
- Observation WC50 increased through germination and early seedling growth in intact tissue and derived protoplasts.
- Observation Before radicle emergence, protoplasts tolerated desiccation less than intact axes.
- Observation After emergence, radicle protoplasts had lower WC50 than intact radicles.
- Intervention effect At 24 h imbibition, 700 mM sucrose improved protoplast dehydration tolerance relative to 500 mM sucrose/raffinose.
- Observation Intact epicotyls generally tolerated desiccation more than radicles.
- Observation Epicotyl and radicle protoplasts did not differ in desiccation tolerance.