Claim · model-written summary
A screen of targeted knockout mutants of Physcomitrella patens identified 20 (0.04 %) auxotrophs.
- Taxon
- Physcomitrella patens
Physcomitrium patense0 - Outcome
- auxotrophse1
- Value
- 20 (0.04 %)e1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid moss Physcomitrella patens”
e1“The screen resulted in the identification of 20 (0.04 %) auxotrophs”
Title
High throughput metabolic screen ofPhyscomitrellatransformants
Julia Schulte; Anika Erxleben; Gabriele Schween; et al. The Bryologist 2006. doi:10.1639/0007-2745(2006)109[247:htmsop]2.0.co;2
Other claims from this paper (10)
- Observation Five auxotrophs showed no growth on minimal medium but were rescued on supplemented medium.
- Observation One vitamin-deficient mutant was identified as a p-aminobenzoic acid auxotroph.
- Observation Three plants were nitrate assimilation deficient mutants.
- Observation One transformant showed undefined growth requirements.
- Observation Two physiological mutants showed an albino phenotype on minimal medium but a green wild type phenotype on supplemented medium.
- Observation Culturing the albino mutants under low light intensities could not prevent bleaching.
- Mechanism The missing chlorophyll production in the albino mutants was not caused by light sensitivity.
- Intervention effect Supplementing the minimal medium with selected compounds of the supplemented medium did not prevent bleaching.
- Observation Both mutants produced green protonemata even when single substances of the supplemented medium were lacking.
- Mechanism A complex network of interactions related to photosynthesis was disturbed in Physcomitrella.