Claim · model-written summary
Five auxotrophs showed no growth on minimal medium but were rescued on supplemented medium.
- Taxon
- Physcomitrella patens
Physcomitrium patense0 - Treatment
- supplemented mediume2
- Outcome
- auxotrophse2
- Value
- fivee2
- Qualifier
- no growth on minimal mediume2
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid moss Physcomitrella patens”
e2“Medium supplementation tests were performed for five auxotrophs, which showed no growth on minimal medium, but were rescued on supplemented medium”
Title
High throughput metabolic screen ofPhyscomitrellatransformants
Julia Schulte; Anika Erxleben; Gabriele Schween; et al. The Bryologist 2006. doi:10.1639/0007-2745(2006)109[247:htmsop]2.0.co;2
Other claims from this paper (10)
- Observation A screen of targeted knockout mutants of Physcomitrella patens identified 20 (0.04 %) auxotrophs.
- Observation One vitamin-deficient mutant was identified as a p-aminobenzoic acid auxotroph.
- Observation Three plants were nitrate assimilation deficient mutants.
- Observation One transformant showed undefined growth requirements.
- Observation Two physiological mutants showed an albino phenotype on minimal medium but a green wild type phenotype on supplemented medium.
- Observation Culturing the albino mutants under low light intensities could not prevent bleaching.
- Mechanism The missing chlorophyll production in the albino mutants was not caused by light sensitivity.
- Intervention effect Supplementing the minimal medium with selected compounds of the supplemented medium did not prevent bleaching.
- Observation Both mutants produced green protonemata even when single substances of the supplemented medium were lacking.
- Mechanism A complex network of interactions related to photosynthesis was disturbed in Physcomitrella.