Claim · model-written summary
Increasing the number of ultrasound exposures during culture usually had negative effects on shikonin yield.
- Taxon
- Lithospermum erythrorhizon
Lithospermum erythrorhizont0 - Treatment
- Increasing in the number of US exposurese0
- Outcome
- shikonin yielde0
- Direction
- decreaseinferrede0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lithospermum erythrorhizon”
e0“Increasing in the number of US exposures during the culture process usually resulted in negative effects on shikonin yield”
Title
Lidong Lin; Jianyong Wu Biotechnology and Bioengineering 2002. doi:10.1002/bit.10180.abs
Other claims from this paper (9)
- Intervention effect Low-energy ultrasound exposure significantly stimulated shikonin biosynthesis in Lithospermum erythrorhizon suspension cells.
- Intervention effect At certain ultrasound doses the volumetric shikonin yield rose by about 60%–70%.
- Intervention effect The share of shikonin excreted from the cells rose from 20% to 65%–70%.
- Mechanism The authors attribute the increased shikonin excretion partly to higher cell membrane permeability caused by sonication.
- Intervention effect Combining ultrasound with in situ organic-solvent extraction (two-phase culture) increased volumetric shikonin yield more than two- to threefold.
- Intervention effect Increasing the number of ultrasound exposures slightly stimulated shikonin excretion.
- Intervention effect Ultrasound at relatively high energy levels caused slight cell growth depression, with at most a 9% decrease in dry cell weight.
- Intervention effect Phenylalanine ammonia lyase and p-hydroxybenzoic acid geranyltransferase, two key enzymes of secondary metabolite biosynthesis, were stimulated by ultrasound.
- Mechanism The authors attribute the ultrasound stimulation of secondary metabolite biosynthesis to cell metabolic activity activated by ultrasound, specifically defense responses to the mechanical stress of irradiation.