Lithospermum erythrorhizon 274 claims

species · family Boraginaceae · green plant · NCBI Taxonomy ↗

Also written in claims: L.erythrorhizon

5 spellings

Questions studied on this organism7 questions8 papers

Lithospermum erythrorhizonQwert1234 · CC BY-SA 4.0 · Wikimedia Commons ↗

274 claims

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Associationobservedoriginal resultmeasured

Claim · model-written summary

PPO genes showed strong expression synchrony with shikonin production.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Gene: polyphenol oxidase (PPO)e0
Outcome: shikonin productione0

Not reportedDirection

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“Genes encoding polyphenol oxidase (PPO), a dicopper-dependent oxidoreductase, was highlighted by the strong synchronous expression with shikonin production”
✓ located verbatim in the source · abstract, characters 729–884

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation LePPO1 was localized in plastids.
Observation Shikonin accumulated in the apoplast.

Associationobservedoriginal resultmeasured

Claim · model-written summary

Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.

Taxon: L. erythrorhizonLithospermum erythrorhizone0
Gene: LePPO1e0
Outcome: shikonin productione0
Value: five PPO genese0

Not reportedDirection

Verbatim

e0
“of the five PPO genes expressed in L. erythrorhizon (LePPOs), only LePPO1 showed a close correlation with shikonin production”
✓ located verbatim in the source · abstract, characters 972–1097

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation LePPO1 was localized in plastids.
Observation Shikonin accumulated in the apoplast.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

LePPO1-edited lines had markedly reduced shikonin content.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Treatment: LePPO1-ge linese0
Gene: LePPO1e0
Outcome: shikonin contente0
Direction: reducede0

Not reportedComparator

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“shikonin content was markedly reduced in all LePPO1-ge lines”
✓ located verbatim in the source · abstract, characters 1307–1367

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation LePPO1 was localized in plastids.
Observation Shikonin accumulated in the apoplast.

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Deoxyshikonofuran content was unchanged in LePPO1-edited lines.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Treatment: LePPO1-ge linese0
Gene: LePPO1e0
Outcome: content of deoxyshikonofurane0
Direction: remained unaffectede0

Not reportedComparator

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“content of deoxyshikonofuran, a hydroquinone-type shunt product that branches after 3''-hydroxygeranylhydroquinone in the shikonin biosynthetic pathway, remained unaffected in the LePPO1-ge lines”
✓ located verbatim in the source · abstract, characters 1379–1574

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation LePPO1 was localized in plastids.
Observation Shikonin accumulated in the apoplast.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors suggest LePPO1 participates in naphthalene ring formation.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Gene: LePPO1e0
Outcome: naphthalene ring formatione0

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“suggest that LePPO1 participates in naphthalene ring formation”
✓ located verbatim in the source · abstract, characters 1670–1732

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Observation LePPO1 was localized in plastids.
Observation Shikonin accumulated in the apoplast.

Observationobservedoriginal resultmeasured

Claim · model-written summary

LePPO1 was localized in plastids.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Organ / stage: plastidse0
Gene: LePPO1e0
Outcome: localized in plastidse0

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“LePPO1 is localized in plastids”
✓ located verbatim in the source · abstract, characters 1749–1780

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation Shikonin accumulated in the apoplast.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Shikonin accumulated in the apoplast.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Organ / stage: apoplaste0
Outcome: shikonin is accumulated in the apoplaste0

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 125–151

e0
“shikonin is accumulated in the apoplast”
✓ located verbatim in the source · abstract, characters 1790–1829

Title

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Kohei Nakanishi; Yuki Takano; Kyoko Yamamoto; et al. PubMed 2026. doi:10.1093/pcp/pcag074

Other claims from this paper (6)

Association PPO genes showed strong expression synchrony with shikonin production.
Association Among five expressed PPO genes, only LePPO1 closely correlated with shikonin production.
Intervention effect LePPO1-edited lines had markedly reduced shikonin content.
Intervention effect Deoxyshikonofuran content was unchanged in LePPO1-edited lines.
Mechanism The authors suggest LePPO1 participates in naphthalene ring formation.
Observation LePPO1 was localized in plastids.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The study identified 176 LeMYB genes.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Gene: LeMYBe1
Outcome: LeMYB genese1
Value: 176e1

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 54–80

e1
“characterizing 176 LeMYB genes”
✓ located verbatim in the source · abstract, characters 425–455

Title

Systematic characterization of the R2R3-MYB family in Lithospermum erythrorhizon and regulatory mechanisms of LeMYB1 in shikonin biosynthesis

Xuan Wang; Yalan Si; Wencai Jie; et al. BMC Plant Biology 2026. doi:10.1186/s12870-026-08283-6

Other claims from this paper (14)

Observation The R2 and R3 domains of LeMYBs were relatively conserved.
Observation LeMYB sequence diversity emerged progressively during evolution.
Observation Phylogenetic analysis classified LeMYBs into 31 subfamilies.
Mechanism Whole-genome duplication mainly expanded the LeMYB subfamilies.
Mechanism Transposed duplication mainly expanded the LeMYB subfamilies.
Mechanism Dispersed duplication mainly expanded the LeMYB subfamilies.
Observation Some LeMYBs had root, leaf or flower-specific expression.
Association LeMYB1 transcript levels were 63-fold higher in roots than leaves.
Intervention effect Shikonin-inducing conditions significantly upregulated LeMYB1.
Observation LeMYB1 was localized in the nucleus.
Observation LeMYB1 bound promoters of LeHMGR2, LeGPPS1 and LePGT1.
Observation LeMYB1 bound promoters of LeGHQ3''H2 and LeSAT1.
Mechanism LeMYB1 activated transcription of the named shikonin biosynthetic genes.
Mechanism LeMYB1 promoted shikonin biosynthesis through transcriptional activation.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The R2 and R3 domains of LeMYBs were relatively conserved.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Gene: LeMYBse1
Outcome: R2 and R3 domains of LeMYBs are relatively conservede1

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 54–80

e1
“the R2 and R3 domains of LeMYBs are relatively conserved”
✓ located verbatim in the source · abstract, characters 511–567

Title

Systematic characterization of the R2R3-MYB family in Lithospermum erythrorhizon and regulatory mechanisms of LeMYB1 in shikonin biosynthesis

Xuan Wang; Yalan Si; Wencai Jie; et al. BMC Plant Biology 2026. doi:10.1186/s12870-026-08283-6

Other claims from this paper (14)

Observation The study identified 176 LeMYB genes.
Observation LeMYB sequence diversity emerged progressively during evolution.
Observation Phylogenetic analysis classified LeMYBs into 31 subfamilies.
Mechanism Whole-genome duplication mainly expanded the LeMYB subfamilies.
Mechanism Transposed duplication mainly expanded the LeMYB subfamilies.
Mechanism Dispersed duplication mainly expanded the LeMYB subfamilies.
Observation Some LeMYBs had root, leaf or flower-specific expression.
Association LeMYB1 transcript levels were 63-fold higher in roots than leaves.
Intervention effect Shikonin-inducing conditions significantly upregulated LeMYB1.
Observation LeMYB1 was localized in the nucleus.
Observation LeMYB1 bound promoters of LeHMGR2, LeGPPS1 and LePGT1.
Observation LeMYB1 bound promoters of LeGHQ3''H2 and LeSAT1.
Mechanism LeMYB1 activated transcription of the named shikonin biosynthetic genes.
Mechanism LeMYB1 promoted shikonin biosynthesis through transcriptional activation.

Observationobservedoriginal resultmeasured

Claim · model-written summary

LeMYB sequence diversity emerged progressively during evolution.

Taxon: Lithospermum erythrorhizonLithospermum erythrorhizont0
Outcome: sequence diversity has progressively emergede1

Verbatim

t0
“Lithospermum erythrorhizon”
✓ located verbatim in the source · title, characters 54–80

e1
“sequence diversity has progressively emerged during evolution”
✓ located verbatim in the source · abstract, characters 575–636

Title

Systematic characterization of the R2R3-MYB family in Lithospermum erythrorhizon and regulatory mechanisms of LeMYB1 in shikonin biosynthesis

Xuan Wang; Yalan Si; Wencai Jie; et al. BMC Plant Biology 2026. doi:10.1186/s12870-026-08283-6

Other claims from this paper (14)

Observation The study identified 176 LeMYB genes.
Observation The R2 and R3 domains of LeMYBs were relatively conserved.
Observation Phylogenetic analysis classified LeMYBs into 31 subfamilies.
Mechanism Whole-genome duplication mainly expanded the LeMYB subfamilies.
Mechanism Transposed duplication mainly expanded the LeMYB subfamilies.
Mechanism Dispersed duplication mainly expanded the LeMYB subfamilies.
Observation Some LeMYBs had root, leaf or flower-specific expression.
Association LeMYB1 transcript levels were 63-fold higher in roots than leaves.
Intervention effect Shikonin-inducing conditions significantly upregulated LeMYB1.
Observation LeMYB1 was localized in the nucleus.
Observation LeMYB1 bound promoters of LeHMGR2, LeGPPS1 and LePGT1.
Observation LeMYB1 bound promoters of LeGHQ3''H2 and LeSAT1.
Mechanism LeMYB1 activated transcription of the named shikonin biosynthetic genes.
Mechanism LeMYB1 promoted shikonin biosynthesis through transcriptional activation.