Claim · model-written summary
PRS4 synthesizes cytosolic PRPP.
- Taxon
- plantsViridiplantaee1
- Gene
- PRS4e0
- Compound
- cytosolic PRPPe0
- Outcome
- synthesizes cytosolic PRPPe0
Not reportedMaterialOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“PRS4 synthesizes cytosolic PRPP, a key upstream metabolite in nucleotide synthesis and salvage pathways.”
e1“TOR activity in plants”
Title
TOR coordinates nucleotide availability with ribosome biogenesis in plants
Michael Busche; M Regina Scarpin; Robert Hnasko; et al. The Plant Cell 2021. doi:10.1093/plcell/koab043
Other claims from this paper (10)
- Method / resource A genetic screen identified PRS4 as a critical TOR regulatory enzyme.
- Observation PRS4 knockouts are embryo lethal in Arabidopsis thaliana.
- Intervention effect Silencing PRS4 in Nicotiana benthamiana caused dwarfism and abnormal leaf shape.
- Intervention effect Silencing PRS4 in Nicotiana benthamiana delayed flowering.
- Observation Ribosome biogenesis was strongly repressed in prs4 knockdowns.
- Intervention effect Chemical disruption of nucleotide biosynthesis inhibited TOR activity.
- Intervention effect Genetic disruption of nucleotide biosynthesis inhibited TOR activity.
- Intervention effect Nucleobase supply reversed TOR inhibition.
- Mechanism TOR transcriptionally promotes nucleotide biosynthesis to support ribosomal RNA synthesis.
- Mechanism The authors propose that TOR coordinates ribosome biogenesis with nucleotide availability.