Claim · model-written summary
Genetic disruption of nucleotide biosynthesis inhibited TOR activity.
- Taxon
- plantsViridiplantaee1
- Treatment
- genetic disruption of nucleotide biosynthesise0
- Outcome
- TOR activitye0
- Direction
- inhibitede0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Verbatim
e0“TOR activity is inhibited by chemical or genetic disruption of nucleotide biosynthesis”
e1“TOR activity in plants”
Title
TOR coordinates nucleotide availability with ribosome biogenesis in plants
Michael Busche; M Regina Scarpin; Robert Hnasko; et al. The Plant Cell 2021. doi:10.1093/plcell/koab043
Other claims from this paper (10)
- Method / resource A genetic screen identified PRS4 as a critical TOR regulatory enzyme.
- Mechanism PRS4 synthesizes cytosolic PRPP.
- Observation PRS4 knockouts are embryo lethal in Arabidopsis thaliana.
- Intervention effect Silencing PRS4 in Nicotiana benthamiana caused dwarfism and abnormal leaf shape.
- Intervention effect Silencing PRS4 in Nicotiana benthamiana delayed flowering.
- Observation Ribosome biogenesis was strongly repressed in prs4 knockdowns.
- Intervention effect Chemical disruption of nucleotide biosynthesis inhibited TOR activity.
- Intervention effect Nucleobase supply reversed TOR inhibition.
- Mechanism TOR transcriptionally promotes nucleotide biosynthesis to support ribosomal RNA synthesis.
- Mechanism The authors propose that TOR coordinates ribosome biogenesis with nucleotide availability.