Vanda bicolor 8 claims

species · family Orchidaceae · green plant · NCBI Taxonomy ↗

Vanda bicolorNational Biodiversity Centre, Serbithang · CC BY 3.0 · Wikimedia Commons ↗

8 claims

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Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Seven-month immature seeds germinated at 88.2% on supplemented MS medium.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Organ / stage: Immature seedse0
Treatment: MS nutrient medium supplemented with 3% sucrose, 3 μM each of NAA and BAe0
Outcome: germinatione0
Value: 88.2%e0

Verbatim

e0
“Immature seeds from green pods of 7 months old registered 88.2% germination on MS nutrient medium supplemented with 3% sucrose, 3 μM each of NAA and BA under normal culture conditions”
✓ located verbatim in the source · abstract, characters 464–647

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Germinating embryos became protocorms and protocorm-like bodies within 55 days.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Organ / stage: germinating embryose0
Outcome: converted to protocorms and protocorm-like bodies (PLBs)e0
Value: 55 dayse0

Verbatim

e0
“The germinating embryos converted to protocorms and protocorm-like bodies (PLBs) on the same germination medium within 55 days of culture”
✓ located verbatim in the source · abstract, characters 649–786

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The supplemented medium supported conversion to plantlets and root development.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Treatment: MS medium fortified with sucrose (3%), NAA and BA (3 + 6 μM respectively) and activated charcoal (0.6%)e0
Outcome: converted to plantlets and supported culture proliferation and simultaneous healthy roots developmente0
Value: 6.2 shootse0

Verbatim

e0
“subsequently converted to plantlets and supported culture proliferation and simultaneous healthy roots development on MS medium fortified with sucrose (3%), NAA and BA (3 + 6 μM respectively) and activated charcoal (0.6%) where as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
✓ located verbatim in the source · abstract, characters 791–1098

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The medium produced 6.2 shoots per subculture per PLB.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Outcome: shoots developed per subculture per PLBse0
Value: 6.2 shootse0

Verbatim

e0
“as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
✓ located verbatim in the source · abstract, characters 1019–1098

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The medium produced 3.3 roots per plant.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Outcome: roots per plante0
Value: 3.3 rootse0

Verbatim

e0
“as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
✓ located verbatim in the source · abstract, characters 1019–1098

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Hardened roots adhered to wood pieces after five to six weeks.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Outcome: velamenous roots started adhering to the wood piecese0
Value: 5–6 wke0

Verbatim

e0
“after 5–6 wk of transfer to the hardening condition, velamenous roots started adhering to the wood pieces”
✓ located verbatim in the source · abstract, characters 1209–1314

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

After direct polyhouse transfer, survival was 98.25% at eight weeks.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Outcome: survival ratee0
Value: 98.25e0
Setting: polyhousee0

Verbatim

e0
“These plants could be transferred to polyhouse as well as in the nature directly where 98.25 and 97.75% survival rate registered respectively after 8 wk of transfer”
✓ located verbatim in the source · abstract, characters 1316–1480

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct transfer to nature, survival was 97.75% at eight weeks.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

After direct transfer to nature, survival was 97.75% at eight weeks.

Taxon: Vanda bicolor Griff.Vanda bicolort0
Outcome: survival ratee0
Value: 97.75%e0
Setting: in the naturee0

Verbatim

e0
“These plants could be transferred to polyhouse as well as in the nature directly where 98.25 and 97.75% survival rate registered respectively after 8 wk of transfer”
✓ located verbatim in the source · abstract, characters 1316–1480

t0
“Vanda bicolor Griff.”
✓ located verbatim in the source · title, characters 36–56

Title

Asymbiotic immature seed culture of Vanda bicolor Griff., plant regeneration and formulation of an efficient new hardening technique for direct field transfer of regenerates

Chitta Ranjan Deb; Aolemla Pongener; Zubenthung P. Kikon South African Journal of Botany 2022. doi:10.1016/j.sajb.2022.08.031

Other claims from this paper (7)

Method / resource Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
Observation Germinating embryos became protocorms and protocorm-like bodies within 55 days.
Method / resource The supplemented medium supported conversion to plantlets and root development.
Method / resource The medium produced 6.2 shoots per subculture per PLB.
Method / resource The medium produced 3.3 roots per plant.
Method / resource Hardened roots adhered to wood pieces after five to six weeks.
Method / resource After direct polyhouse transfer, survival was 98.25% at eight weeks.