By organism › Green plants › Fagaceae › Castanea sativa

Observationobservedoriginal resultmeasured

Claim · model-written summary

Only line CI-9 produced kanamycin-resistant transgenic embryos, with a 5.9% transformation efficiency.

Taxon: Castanea sativaCastanea sativae0
Material: line CI-9e1
Outcome: transformation efficiencye1
Value: 5.9%e1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“Only line CI-9 produced kanamycin resistant transgenic embryos, with an average transformation efficiency of 5.9%”
✓ located verbatim in the source · abstract, characters 845–958

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Editing was confirmed in five of seven embryogenic chestnut lines.

Taxon: Castanea sativaCastanea sativae0
Outcome: Editinge1
Value: five of sevene1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“Editing was confirmed in five of seven embryogenic lines”
✓ located verbatim in the source · abstract, characters 1135–1191

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

All five edited lines have at least 97% editing efficiency in at least one gRNA.

Taxon: Castanea sativaCastanea sativae0
Outcome: editing efficiencye1
Value: at least 97%e1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“All five lines have at least 97% editing efficiency in at least one gRNA”
✓ located verbatim in the source · abstract, characters 1193–1265

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Edited somatic embryos had survival rates exceeding 50% in three of the analyzed lines.

Taxon: Castanea sativaCastanea sativae0
Organ / stage: Edited somatic embryose1
Outcome: survival ratese1
Value: exceeding 50%e1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“Edited somatic embryos showed the highest survival rates, exceeding 50% in three of the analyzed lines”
✓ located verbatim in the source · abstract, characters 1267–1369

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Knockout of pmr4 in regenerated plants of line CI-9 reduced root necrosis to 8%, from 57% in wild-type explants.

Taxon: Castanea sativaCastanea sativae0
Material: line CI-9- pmr4 -1e1
Treatment: Targeted editing of the Cspmr4 gene via CRISPR/Cas9t0
Interactor: Phytophthora cinnamomiPhytophthora cinnamomie2pathogen
Gene: pmr4e1
Outcome: root necrosise1
Direction: decreasinge1
Value: 8%e1
Qualifier: 57% in wild-type explantse1
Comparator: wild-type explantse1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“regenerated plants from line CI-9- pmr4 -1 exhibited a marked reduction in root necrosis, decreasing from 57% in wild-type explants to only 8%”
✓ located verbatim in the source · abstract, characters 1384–1526

e2
“ink disease and chestnut blight, caused respectively by Phytophthora cinnamomi (Pc)”
✓ located verbatim in the source · abstract, characters 177–260

t0
“Targeted editing of the Cspmr4 gene via CRISPR/Cas9”
✓ located verbatim in the source · title, characters 0–51

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

The susceptibility gene Cspmr4 was knocked out for the first time in a forest species.

Taxon: Castanea sativaCastanea sativae0
Gene: Cspmr4e1
Outcome: knocked-oute1

Verbatim

e0
“Castanea sativa Mill. is a highly versatile species”
✓ located verbatim in the source · abstract, characters 9–60

e1
“the susceptibility gene Cspmr4, which encodes a callose synthase, was knocked-out for the first time in a forest species using CRISPR/Cas9”
✓ located verbatim in the source · abstract, characters 492–630

Title

Targeted editing of the Cspmr4 gene via CRISPR/Cas9 to enhance tolerance to Phytophthora cinnamomi in Castanea sativa

Vera Pavese; María Teresa Martínez; Pablo Piñeiro; et al. Plant Stress 2026. doi:10.1016/j.stress.2026.101376

Other claims from this paper (5)

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Bacillus velezensis N1 significantly inhibited the growth of Gnomoniopsis smithogilvyi (G. smithogilvyi) in vitro.

Taxon: Castanea sativaCastanea sativae1
Treatment: B. velezensis strain N1e0
Interactor: G. smithogilvyiGnomoniopsis smithogilvyie0pathogen
Outcome: growthe0
Direction: inhibitede0

Not reportedComparator

Verbatim

e0
“Moreover, B. velezensis strain N1 significantly inhibited the growth of N. parvum and G. smithogilvyi and showed a biocontrol efficacy on detached eucalyptus and chestnut shoots”
✓ located verbatim in the source · abstract, characters 957–1134

e1
“causal agents of canker diseases on Eucalyptus globulus and Castanea sativa”
✓ located verbatim in the source · abstract, characters 338–413

Title

Comprehensive Genomic Analysis and Evaluation of In Vivo and In Vitro Biocontrol Efficacy of Bacillus velezensis N1 Against Gnomoniopsis smithogilvyi and Neofusicoccum parvum

Anna Maria Vettraino; Michele Narduzzi; Benedetto Teodoro Linaldeddu; et al. Forests 2026. doi:10.3390/f17040418

Other claims from this paper (8)

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In preventive treatment, applying Bacillus velezensis N1 significantly reduced the length of necrotic lesions compared with pathogen-only controls on Eucalyptus globulus and Castanea sativa.

Taxon: Eucalyptus globulus and Castanea sativaCastanea sativaEucalyptus globuluse1
Treatment: application of B. velezensis N1e2
Outcome: length of necrotic lesionse2
Direction: reductione2
Qualifier: preventivee2
Comparator: pathogen-only controlse2

Verbatim

e1
“causal agents of canker diseases on Eucalyptus globulus and Castanea sativa”
✓ located verbatim in the source · abstract, characters 338–413

e2
“In both preventive and curative treatments and pathosystems, the application of B. velezensis N1 resulted in a significant reduction in the length of necrotic lesions, compared to pathogen-only controls”
✓ located verbatim in the source · abstract, characters 1136–1338

Title

Comprehensive Genomic Analysis and Evaluation of In Vivo and In Vitro Biocontrol Efficacy of Bacillus velezensis N1 Against Gnomoniopsis smithogilvyi and Neofusicoccum parvum

Anna Maria Vettraino; Michele Narduzzi; Benedetto Teodoro Linaldeddu; et al. Forests 2026. doi:10.3390/f17040418

Other claims from this paper (8)

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In curative treatment, applying Bacillus velezensis N1 significantly reduced the length of necrotic lesions compared with pathogen-only controls on Eucalyptus globulus and Castanea sativa.

Taxon: Eucalyptus globulus and Castanea sativaCastanea sativaEucalyptus globuluse1
Treatment: application of B. velezensis N1e2
Outcome: length of necrotic lesionse2
Direction: reductione2
Qualifier: curativee2
Comparator: pathogen-only controlse2

Verbatim

e1
“causal agents of canker diseases on Eucalyptus globulus and Castanea sativa”
✓ located verbatim in the source · abstract, characters 338–413

e2
“In both preventive and curative treatments and pathosystems, the application of B. velezensis N1 resulted in a significant reduction in the length of necrotic lesions, compared to pathogen-only controls”
✓ located verbatim in the source · abstract, characters 1136–1338

Title

Comprehensive Genomic Analysis and Evaluation of In Vivo and In Vitro Biocontrol Efficacy of Bacillus velezensis N1 Against Gnomoniopsis smithogilvyi and Neofusicoccum parvum

Anna Maria Vettraino; Michele Narduzzi; Benedetto Teodoro Linaldeddu; et al. Forests 2026. doi:10.3390/f17040418

Other claims from this paper (8)

Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No phytotoxic effects were observed on shoots treated with Bacillus velezensis N1.

Taxon: Eucalyptus globulus and Castanea sativaCastanea sativaEucalyptus globuluse1
Organ / stage: treated shootse3
Outcome: phytotoxic effectse3

Verbatim

e1
“causal agents of canker diseases on Eucalyptus globulus and Castanea sativa”
✓ located verbatim in the source · abstract, characters 338–413

e3
“while no phytotoxic effects were observed on treated shoots”
✓ located verbatim in the source · abstract, characters 1340–1399

Title

Comprehensive Genomic Analysis and Evaluation of In Vivo and In Vitro Biocontrol Efficacy of Bacillus velezensis N1 Against Gnomoniopsis smithogilvyi and Neofusicoccum parvum

Anna Maria Vettraino; Michele Narduzzi; Benedetto Teodoro Linaldeddu; et al. Forests 2026. doi:10.3390/f17040418

Other claims from this paper (8)

Castanea sativa 1,655 claims

1,655 claims