Abstract
Phosphorus deficiency results in the formation of longer root hairs in Brassica carinata cv. Bale to exhaust a greater soil volume. Recently, suppression subtractive hybridization revealed genes that were regulated in P-starved roots in Brassica carinata. Sequencing identified genes sharing homologies to a leucine-rich repeat protein kinase (LRR), a transcription factor of the 14-3-3 family, and a proline rich family protein (PRP) in Arabidopsis thaliana. This study aims at characterizing the expression pattern of these putative P-responsive genes by specifying the response to several nutritional stress factors and by inducing or discontinuing phosphorus deprivation. Root hair length was enhanced with phosphorus and nitrogen deprivation but not with potassium depletion. Likewise, the transcription level of LRR and PRP was increased in -P and -N conditions whereas there was no clear response for 14-3-3. One day after varying P supply, phenotypically the root hair length was changed. Correspondingly, the expression of the root-specific LRR and PRP was induced by P-deficiency and inhibited by the addition of P. The response to P starvation of 14-3-3 followed no clear trend. P-resupply increased the gene expression as well as P removal with a short term repression after 3 hours. In summary, the expression of a LRR receptor-like protein kinase and a proline rich protein suggests a function of these genes in inducing root hair growth. The putative transcription factor 14-3-3 may be of importance in rapid but unspecific stress response.
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Claims 14
Claim · model-written summary
Phosphorus and nitrogen deprivation enhanced root hair length, while potassium depletion did not.
- Taxon
- Brassica carinata
Brassica carinatae1 - Material
- cv. Balee1
- Treatment
- phosphorus and nitrogen deprivatione0
- Outcome
- Root hair lengthe0
- Direction
- enhancede0
- Comparator
- potassium depletione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root hair length was enhanced with phosphorus and nitrogen deprivation but not with potassium depletion”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
LRR transcription increased under phosphorus and nitrogen deprivation.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- -P and -N conditionse0
- Gene
- LRRe0
- Outcome
- transcription level of LRRe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription level of LRR and PRP was increased in -P and -N conditions”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
PRP transcription increased under phosphorus and nitrogen deprivation.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- -P and -N conditionse0
- Gene
- PRPe0
- Outcome
- transcription level of LRR and PRPe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription level of LRR and PRP was increased in -P and -N conditions”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
The 14-3-3 transcription response to nutrient deprivation was unclear.
- Taxon
- Brassica carinataBrassica carinatae1
- Gene
- 14-3-3e0
- Outcome
- no clear response for 14-3-3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no clear response for 14-3-3”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Root hair length changed one day after phosphorus supply varied.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- varying P supplye0
- Outcome
- root hair lengthe0
- Qualifier
- One daye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One day after varying P supply, phenotypically the root hair length was changed”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus deficiency induced root-specific LRR expression.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- P-deficiencye0
- Gene
- LRRe0
- Outcome
- expression of the root-specific LRRe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the root-specific LRR and PRP was induced by P-deficiency and inhibited by the addition of P”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus deficiency induced root-specific PRP expression.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- P-deficiencye0
- Gene
- PRPe0
- Outcome
- expression of the root-specific LRR and PRPe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the root-specific LRR and PRP was induced by P-deficiency and inhibited by the addition of P”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus addition inhibited root-specific LRR expression.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- addition of Pe0
- Gene
- LRRe0
- Outcome
- expression of the root-specific LRRe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the root-specific LRR and PRP was induced by P-deficiency and inhibited by the addition of P”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus addition inhibited root-specific PRP expression.
- Taxon
- Brassica carinataBrassica carinatae1
- Treatment
- addition of Pe0
- Gene
- PRPe0
- Outcome
- expression of the root-specific LRR and PRPe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the root-specific LRR and PRP was induced by P-deficiency and inhibited by the addition of P”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
The 14-3-3 response to phosphorus starvation showed no clear trend.
- Taxon
- Brassica carinataBrassica carinatae1
- Gene
- 14-3-3e0
- Outcome
- response to P starvation of 14-3-3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response to P starvation of 14-3-3 followed no clear trend”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus resupply increased 14-3-3 gene expression.
- Taxon
- Brassica carinataBrassica carinatae2
- Treatment
- P-resupplye0
- Gene
- 14-3-3e1
- Outcome
- gene expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P-resupply increased the gene expression as well as P removal with a short term repression after 3 hours”
e1“The response to P starvation of 14-3-3 followed no clear trend”
e2“Brassica carinata cv. Bale”
Claim · model-written summary
Phosphorus removal increased 14-3-3 gene expression after short term repression at three hours.
- Taxon
- Brassica carinataBrassica carinatae2
- Treatment
- P removale0
- Gene
- 14-3-3e1
- Outcome
- gene expressione0
- Direction
- increasede0
- Qualifier
- short term repression after 3 hourse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P-resupply increased the gene expression as well as P removal with a short term repression after 3 hours”
e1“The response to P starvation of 14-3-3 followed no clear trend”
e2“Brassica carinata cv. Bale”
Claim · model-written summary
The authors suggest LRR and PRP function in inducing root hair growth.
- Taxon
- Brassica carinataBrassica carinatae1
- Gene
- LRR receptor-like protein kinasee0
- Outcome
- root hair growthe0
- Qualifier
- suggests a function of these genes in inducing root hair growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of a LRR receptor-like protein kinase and a proline rich protein suggests a function of these genes in inducing root hair growth”
e1“Brassica carinata cv. Bale”
Claim · model-written summary
The authors suggest 14-3-3 may contribute to a rapid, nonspecific stress response.
- Taxon
- Brassica carinataBrassica carinatae1
- Gene
- 14-3-3e0
- Outcome
- rapid but unspecific stress responsee0
- Qualifier
- may be of importancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The putative transcription factor 14-3-3 may be of importance in rapid but unspecific stress response”
e1“Brassica carinata cv. Bale”
The same questions in other papers
nitrogen · growth · Brassica carinata (Ethiopian mustard)Positive and negative results
4 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.