Abstract
NADPH oxidases (also known as respiratory burst oxidase homologs, Rbohs) are key enzymes that catalyze the generation of reactive oxygen species (ROS) in plants. In the present study, eight SlRboh genes were identified in tomato and their possible involvement in resistance to Botrytis cinerea and drought tolerance was examined. Expression of SlRbohs was induced by B. cinerea and Pseudomonas syringae pv. tomato but displayed distinct patterns. Virus-induced gene silencing based silencing of SlRbohB resulted in reduced resistance to B. cinerea but silencing of other SlRbohs did not affect the resistance. Compared to non-silenced plants, the SlRbohB-silenced plants accumulated more ROS and displayed attenuated expression of defense genes after infection with B. cinerea. Silencing of SlRbohB also suppressed flg22-induced ROS burst and the expression of SlLrr22, a marker gene related to PAMP-triggered immunity (PTI). Transient expression of SlRbohB in Nicotiana benthamiana led to enhanced resistance to B. cinerea. Furthermore, silencing of SlRbohB resulted in decreased drought tolerance, accelerated water loss in leaves and the altered expression of drought-responsive genes. Our data demonstrate that SlRbohB positively regulates the resistance to B. cinerea, flg22-induced PTI, and drought tolerance in tomato.
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Claims 12
Claim · model-written summary
Eight SlRboh genes were identified in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- SlRbohe0
- Outcome
- genes were identifiede0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight SlRboh genes were identified in tomato”
Claim · model-written summary
B. cinerea and Pseudomonas syringae pv. tomato induced distinct SlRboh expression patterns.
- Treatment
- B. cinerea and Pseudomonas syringae pv. tomatoe0
- Gene
- SlRbohse0
- Outcome
- Expression of SlRbohse0
- Direction
- inducede0
Not reportedTaxonComparator
Verbatim
e0“Expression of SlRbohs was induced by B. cinerea and Pseudomonas syringae pv. tomato but displayed distinct patterns”
Claim · model-written summary
Silencing SlRbohB reduced resistance to B. cinerea.
- Treatment
- silencing of SlRbohBe0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Gene
- SlRbohBe0
- Outcome
- resistance to B. cinereae0
- Direction
- reducede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SlRbohB resulted in reduced resistance to B. cinerea”
Claim · model-written summary
Silencing other SlRbohs did not affect B. cinerea resistance.
Not reportedTaxonComparator
Verbatim
e0“silencing of other SlRbohs did not affect the resistance”
Claim · model-written summary
SlRbohB-silenced plants accumulated more ROS after B. cinerea infection.
- Treatment
- SlRbohB-silenced plantse0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- ROSe0
- Direction
- moree0
- Comparator
- non-silenced plantse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to non-silenced plants, the SlRbohB-silenced plants accumulated more ROS and displayed attenuated expression of defense genes after infection with B. cinerea”
Claim · model-written summary
SlRbohB-silenced plants had attenuated defense gene expression after infection.
- Treatment
- SlRbohB-silenced plantse0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- expression of defense genese0
- Direction
- attenuatede0
- Comparator
- non-silenced plantse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to non-silenced plants, the SlRbohB-silenced plants accumulated more ROS and displayed attenuated expression of defense genes after infection with B. cinerea”
Claim · model-written summary
Silencing SlRbohB suppressed the flg22-induced ROS burst.
Not reportedTaxonComparator
Verbatim
e0“Silencing of SlRbohB also suppressed flg22-induced ROS burst and the expression of SlLrr22”
Claim · model-written summary
Silencing SlRbohB suppressed SlLrr22 expression.
Not reportedTaxonComparator
Verbatim
e0“Silencing of SlRbohB also suppressed flg22-induced ROS burst and the expression of SlLrr22”
Claim · model-written summary
Transient expression of SlRbohB enhanced B. cinerea resistance in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Transient expression of SlRbohBe0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- SlRbohBe0
- Outcome
- resistance to B. cinereae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of SlRbohB in Nicotiana benthamiana led to enhanced resistance to B. cinerea”
Claim · model-written summary
Silencing SlRbohB decreased drought tolerance.
Not reportedTaxonComparator
Verbatim
e0“silencing of SlRbohB resulted in decreased drought tolerance, accelerated water loss in leaves and the altered expression of drought-responsive genes”
Claim · model-written summary
Silencing SlRbohB accelerated water loss in leaves.
Not reportedTaxonComparator
Verbatim
e0“silencing of SlRbohB resulted in decreased drought tolerance, accelerated water loss in leaves and the altered expression of drought-responsive genes”
Claim · model-written summary
Silencing SlRbohB altered drought-responsive gene expression.
Not reportedTaxonComparator
Verbatim
e0“silencing of SlRbohB resulted in decreased drought tolerance, accelerated water loss in leaves and the altered expression of drought-responsive genes”
The same questions in other papers
transgenic · Botrytis cinerea · Nicotiana benthamianaPositive and negative results
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.