Abstract
Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.
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Claims 7
Claim · model-written summary
A 1.3-kb dehydrin cDNA was cloned from drought-stressed maize scutellums.
- Organ / stage
- 5-day-old germinating maize scutellumse0
- Treatment
- drought stresse0
- Outcome
- cDNAe0
- Value
- 1.3-kbe0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress”
Claim · model-written summary
The deduced sequence encoded an SK LEA protein and was named ZmDhn2.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced protein sequence indicated a dehydrin gene encoding SK LEA protein”
e1“it was named maize DEHYDRIN2 (ZmDhn2)”
Claim · model-written summary
ZmDhn2 accumulated rapidly and highly in drought-stressed scutellum and leaf tissues.
- Organ / stage
- scutellum and leaf tissuese0
- Treatment
- drought-stressede0
- Gene
- ZmDhn2e1
- Outcome
- accumulatese0
- Direction
- rapidly and highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage”
e1“maize DEHYDRIN2 (ZmDhn2)”
Claim · model-written summary
ZmDhn2 did not accumulate under cold stress.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not under cold stress”
e1“maize DEHYDRIN2 (ZmDhn2)”
Claim · model-written summary
Under normal growth, electrolyte leakage did not differ significantly between Arabidopsis wild type and transformants.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- transformantse0
- Outcome
- differencee0
- Direction
- no significant differencee0
- Qualifier
- normal growth conditione0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant difference showed between wild type and transformants under normal growth condition”
e1“Arabidopsis thaliana”
Claim · model-written summary
Under drought, wild type Arabidopsis had about three times the electrolyte leakage of transformants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- transformed plantse0
- Outcome
- leakage level of electrolytee0
- Direction
- as high ase0
- Value
- 3 timese0
- Qualifier
- under drought stresse0
- Comparator
- wild type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress”
e1“Arabidopsis thaliana”
Claim · model-written summary
ZmDHN2 may increase drought tolerance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It suggests that ZmDHN2 playa role in increasing drought tolerance”
t0“Maize”