Abstract
We have identified a class of small mRNAs (approximately 0.5 kilobases), referred to as small auxin-up RNAs (SAURs), that increases in abundance within minutes after auxin application to excised elongating hypocotyl sections of soybean. In this study, we present evidence that SAURs accumulate in the absence of auxin when protein synthesis is inhibited. Superinduction of SAURs occurs if the synthetic auxin 2,4-dichlorophenoxyacetic acid is added under conditions where protein synthesis is inhibited. Transcription run-on experiments with isolated nuclei show that, unlike 2,4-dichlorophenoxyacetic acid, protein synthesis inhibitors do not activate transcription of the SAUR genes. These results suggest that protein synthesis inhibitors act by stabilizing SAURs and that some labile protein(s) are involved in the rapid turnover of SAURs. This stabilization is not observed with GH3, another auxin-inducible mRNA. Whether induced with 2,4-dichlorophenoxyacetic acid or cycloheximide, SAURs are primarily expressed in epidermal and cortical cells of elongating hypocotyl sections, with little or no expression in vascular tissue. Unlike 2,4-dichlorophenoxyacetic acid-induced SAUR accumulation, the increase in abundance of SAURs induced by cycloheximide is transient, with a peak approximately 1 h after inhibitor addition. Complete inhibition of protein synthesis is not required for SAUR accumulation in the presence of protein synthesis inhibitors.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
SAUR abundance rises within minutes after auxin application.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- elongating hypocotyl sectionse0
- Treatment
- auxin applicatione0
- Gene
- small auxin-up RNAs (SAURs)e0
- Outcome
- abundancee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“small auxin-up RNAs (SAURs), that increases in abundance within minutes after auxin application to excised elongating hypocotyl sections of soybean”
Claim · model-written summary
SAURs accumulated without auxin under inhibited protein synthesis.
Not reportedTaxonDirection
Verbatim
e0“SAURs accumulate in the absence of auxin when protein synthesis is inhibited”
Claim · model-written summary
Adding 2,4-dichlorophenoxyacetic acid during protein synthesis inhibition superinduced SAURs.
Not reportedTaxonComparator
Verbatim
e0“Superinduction of SAURs occurs if the synthetic auxin 2,4-dichlorophenoxyacetic acid is added under conditions where protein synthesis is inhibited”
Claim · model-written summary
Protein synthesis inhibitors did not activate SAUR-gene transcription unlike the synthetic auxin.
- Treatment
- protein synthesis inhibitorse0
- Gene
- SAUR genese0
- Outcome
- transcription of the SAUR genese0
- Direction
- do not activatee0
- Comparator
- 2,4-dichlorophenoxyacetic acide0
Not reportedTaxon
Verbatim
e0“unlike 2,4-dichlorophenoxyacetic acid, protein synthesis inhibitors do not activate transcription of the SAUR genes”
Claim · model-written summary
The authors infer that inhibitors stabilize SAURs.
Not reportedTaxon
Verbatim
e0“protein synthesis inhibitors act by stabilizing SAURs”
Claim · model-written summary
The authors infer a labile protein participates in rapid SAUR turnover.
Not reportedTaxon
Verbatim
e0“some labile protein(s) are involved in the rapid turnover of SAURs”
Claim · model-written summary
GH3 did not show this stabilization.
Not reportedTaxon
Verbatim
e0“This stabilization is not observed with GH3”
Claim · model-written summary
SAURs were primarily expressed in epidermal and cortical cells, with little vascular expression.
- Organ / stage
- epidermal and cortical cells of elongating hypocotyl sectionse0
- Gene
- SAURse0
- Outcome
- primarily expressede0
Not reportedTaxon
Verbatim
e0“SAURs are primarily expressed in epidermal and cortical cells of elongating hypocotyl sections, with little or no expression in vascular tissue”
Claim · model-written summary
Cycloheximide-induced SAUR increase was transient and peaked at about one hour.
- Treatment
- cycloheximidee0
- Gene
- SAURse0
- Outcome
- abundance of SAURse0
- Direction
- increasee0
- Value
- approximately 1 he0
Not reportedTaxonComparator
Verbatim
e0“the increase in abundance of SAURs induced by cycloheximide is transient, with a peak approximately 1 h after inhibitor addition”
Claim · model-written summary
Complete protein synthesis inhibition was unnecessary for SAUR accumulation.
Not reportedTaxon
Verbatim
e0“Complete inhibition of protein synthesis is not required for SAUR accumulation in the presence of protein synthesis inhibitors”
The same questions in other papers
class · pest · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.