Abstract
Potato (Solanum tuberosum L.) cells were transformed with an antisense DNA construct encoding part of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15), the first enzyme of the shikimate pathway, to examine the role(s) of this protein in plant growth and development. Chimeric DNA constructs contained the transcript start site, the first exon, and part of the first intron of the shkA gene in antisense or sense orientations under the control of the cauliflower mosaic virus 35S promoter. Some, but not all, of the transgenic plants expressing antisense DAHP synthase RNA showed reduced levels of wound-induced DAHP synthase enzyme activity, polypeptide, and mRNA 12 and 24 h after wounding. No alteration in the wound induction of DAHP synthase gene expression was observed in transgenic potato tubers containing the chimeric sense construct. Reduced steady-state levels of DAHP synthase mRNA were observed in stem and shoot tip tissue. Some plants with the chimeric antisense construct had reduced stem length, stem diameter, and reduced stem lignification.
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Claims 6
Claim · model-written summary
Some antisense plants had reduced wound-induced DAHP synthase activity at 12 and 24 hours.
- Taxon
- Potato
Solanum tuberosume1 - Treatment
- expressing antisense DAHP synthase RNAe0
- Gene
- DAHP synthasee0
- Outcome
- DAHP synthase enzyme activitye0
- Direction
- reducede0
- Qualifier
- 12 and 24 h after woundinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some, but not all, of the transgenic plants expressing antisense DAHP synthase RNA showed reduced levels of wound-induced DAHP synthase enzyme activity, polypeptide, and mRNA 12 and 24 h after wounding.”
e1“Potato (Solanum tuberosum L.) cells”
Claim · model-written summary
Some antisense plants had reduced wound-induced DAHP synthase polypeptide and mRNA.
- Taxon
- PotatoSolanum tuberosume1
- Treatment
- expressing antisense DAHP synthase RNAe0
- Gene
- DAHP synthasee0
- Outcome
- polypeptide, and mRNAe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some, but not all, of the transgenic plants expressing antisense DAHP synthase RNA showed reduced levels of wound-induced DAHP synthase enzyme activity, polypeptide, and mRNA 12 and 24 h after wounding.”
e1“Potato (Solanum tuberosum L.) cells”
Claim · model-written summary
The sense construct did not alter wound induction of DAHP synthase expression in tubers.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Treatment
- chimeric sense constructe0
- Gene
- DAHP synthasee0
- Outcome
- wound induction of DAHP synthase gene expressione0
- Direction
- No alteratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No alteration in the wound induction of DAHP synthase gene expression was observed in transgenic potato tubers containing the chimeric sense construct.”
Claim · model-written summary
Steady-state DAHP synthase mRNA was reduced in stem and shoot tip tissue.
- Taxon
- PotatoSolanum tuberosume1
- Organ / stage
- stem and shoot tip tissuee0
- Gene
- DAHP synthasee0
- Outcome
- steady-state levels of DAHP synthase mRNAe0
- Direction
- Reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced steady-state levels of DAHP synthase mRNA were observed in stem and shoot tip tissue.”
e1“Potato (Solanum tuberosum L.) cells”
Claim · model-written summary
Some antisense plants had reduced stem length and diameter.
- Taxon
- PotatoSolanum tuberosume1
- Treatment
- chimeric antisense constructe0
- Outcome
- stem length, stem diametere0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some plants with the chimeric antisense construct had reduced stem length, stem diameter, and reduced stem lignification.”
e1“Potato (Solanum tuberosum L.) cells”
Claim · model-written summary
Some antisense plants had reduced stem lignification.
- Taxon
- PotatoSolanum tuberosume1
- Treatment
- chimeric antisense constructe0
- Outcome
- stem lignificatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some plants with the chimeric antisense construct had reduced stem length, stem diameter, and reduced stem lignification.”
e1“Potato (Solanum tuberosum L.) cells”
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