Paper · 2012 · Scholarworks (University of Massachusetts Amherst)

Impact of Ascorbic Acid on Seed Germination, Seedling Growth, and Enzyme Activity of Salt-Stressed Fenugreek

Behairy, Rehab Tawdy

8
claims
14
verbatim quotes
24
citations
1
research areas
✓
Open access

Abstract

Fenugreek (Trigonella foenum-graecum) seeds soaked in ascorbic acid had increased germination, seedling shoot length and total chlorophyll under salt stress as compared with seeds not treated with ascorbic acid. Root length, fresh weight, dry weight, proline, and catalase activity (CAT) increased in salt stressed seedling in which seeds were not treated with ascorbic acid. In seeds treated with ascorbic acid, the salt stress effect on CAT activity was decreased. Ascorbic acid pretreatment of seeds counteracted the decrease in ascorbate oxidase (AO) induced by salt stress, but appeared to act synergistically with salt stress to decrease proline dehydrogenase. The application of ascorbic acid to fenugreek seed apparently increased antioxidant activity, leading to an increase in resistance to salt stress. Salt stress and the ascorbic acid treatment of seeds led to metabolic changes in seedlings as evidenced by changes in peroxidase (Prx) and esterase (Est) isozymes associated with increases in salt and ascorbic acid concentrations. Such changes could account for increases in seedling vigor with ascorbic acid seed treatment and the ability of the seedling to grow in the presence of a salt stress.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ascorbic acid-soaked fenugreek seeds had increased germination, shoot length and total chlorophyll under salt stress.

Treatment
seeds soaked in ascorbic acide1
Outcome
germination, seedling shoot length and total chlorophylle1
Direction
increasede1
Qualifier
under salt stresse1
Comparator
seeds not treated with ascorbic acide1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Fenugreek (Trigonella foenum-graecum) seeds soaked in ascorbic acid had increased germination, seedling shoot length and total chlorophyll under salt stress as compared with seeds not treated with ascorbic acid”

✓ located verbatim in the source · abstract, characters 0–210 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Salt-stressed seedlings from untreated seeds had increased root length, fresh weight and dry weight.

Treatment
salt stressed seedlinge2
Outcome
Root length, fresh weight, dry weighte2
Direction
increasede2
Comparator
seeds were not treated with ascorbic acide2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e2

“Root length, fresh weight, dry weight, proline, and catalase activity (CAT) increased in salt stressed seedling in which seeds were not treated with ascorbic acid”

✓ located verbatim in the source · abstract, characters 212–374 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Salt-stressed seedlings from untreated seeds had increased proline and catalase activity (CAT).

Treatment
salt stressed seedlinge2
Outcome
proline, and catalase activity (CAT)e2
Direction
increasede2
Comparator
seeds were not treated with ascorbic acide2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e2

“Root length, fresh weight, dry weight, proline, and catalase activity (CAT) increased in salt stressed seedling in which seeds were not treated with ascorbic acid”

✓ located verbatim in the source · abstract, characters 212–374 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ascorbic acid seed treatment decreased the salt stress effect on CAT activity.

Treatment
seeds treated with ascorbic acide3
Outcome
salt stress effect on CAT activitye3
Direction
decreasede3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e3

“In seeds treated with ascorbic acid, the salt stress effect on CAT activity was decreased”

✓ located verbatim in the source · abstract, characters 376–465 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ascorbic acid pretreatment counteracted the salt-induced decrease in ascorbate oxidase (AO) activity.

Treatment
Ascorbic acid pretreatment of seedse4
Gene
ascorbate oxidase (AO)e4
Outcome
decrease in ascorbate oxidase (AO) induced by salt stresse4
Direction
counteractede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e4

“Ascorbic acid pretreatment of seeds counteracted the decrease in ascorbate oxidase (AO) induced by salt stress, but appeared to act synergistically with salt stress to decrease proline dehydrogenase”

✓ located verbatim in the source · abstract, characters 467–665 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Ascorbic acid pretreatment appeared to act synergistically with salt stress to decrease proline dehydrogenase.

Treatment
Ascorbic acid pretreatment of seedse4
Gene
proline dehydrogenasee4
Outcome
proline dehydrogenasee4
Direction
decreasee4
Qualifier
synergistically with salt stresse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e4

“Ascorbic acid pretreatment of seeds counteracted the decrease in ascorbate oxidase (AO) induced by salt stress, but appeared to act synergistically with salt stress to decrease proline dehydrogenase”

✓ located verbatim in the source · abstract, characters 467–665 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Ascorbic acid application apparently increased antioxidant activity, leading to increased resistance to salt stress in fenugreek.

Treatment
application of ascorbic acid to fenugreek seede5
Outcome
resistance to salt stresse5
Qualifier
increased antioxidant activitye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“The application of ascorbic acid to fenugreek seed apparently increased antioxidant activity, leading to an increase in resistance to salt stress”

✓ located verbatim in the source · abstract, characters 667–812 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Salt stress and ascorbic acid seed treatment changed peroxidase (Prx) and esterase (Est) isozymes in seedlings.

Treatment
Salt stress and the ascorbic acid treatment of seedse6
Outcome
changes in peroxidase (Prx) and esterase (Est) isozymese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trigonella foenum-graecum
Trigonella foenum-graecum · TaxonYak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Salt-Stressed Fenugreek”

✓ located verbatim in the source · title, characters 85–108 · show in text ↑
e6

“Salt stress and the ascorbic acid treatment of seeds led to metabolic changes in seedlings as evidenced by changes in peroxidase (Prx) and esterase (Est) isozymes”

✓ located verbatim in the source · abstract, characters 814–976 · show in text ↑

application · physiology: pigments · Trigonella foenum-graecum (fenugreek)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.