Paper · 1980 · Plant and Cell Physiology

The exudation and characterization of iron-solubilizing compounds in a rice cell suspension culture

Kunihiko Ojima; Koji Ohira

7
claims
12
verbatim quotes
2
citations
1
research areas
—
Not open access

Abstract

Some compounds capable of solubilizing ferric chloride as the iron source were released into the growth medium from cultured rice cells in the latter half of the growth cycle. This exudation of iron-solubilizing compounds was repressed by an addition of ammonium ion, which, of five cations, was taken up most rapidly. These iron-solubilizing compounds from cultured rice cells were identified by chromatography to be principally malic acid and some citric acid. In the medium containing almost insoluble ferric chloride as the iron source, the growth rate of the cultured cells decreased when cells were inoculated at a low population density. This iron deficiency at the low initial population was rectified by an addition of a small amount of conditioned medium or malic acid.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Cultured rice cells released iron-solubilizing compounds in the latter half of the growth cycle.

Organ / stage
cultured rice cellse0
Outcome
compounds capable of solubilizing ferric chloridee0
Qualifier
latter half of the growth cyclee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Some compounds capable of solubilizing ferric chloride as the iron source were released into the growth medium from cultured rice cells in the latter half of the growth cycle.”

✓ located verbatim in the source · abstract, characters 0–175 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Adding ammonium ion repressed exudation of iron-solubilizing compounds.

Treatment
addition of ammonium ione0
Outcome
exudation of iron-solubilizing compoundse0
Direction
repressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice cell suspension culture”

✓ located verbatim in the source · title, characters 71–99 · show in text ↑
e0

“This exudation of iron-solubilizing compounds was repressed by an addition of ammonium ion”

✓ located verbatim in the source · abstract, characters 176–266 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ammonium ion was taken up most rapidly among five cations.

Organ / stage
rice cell suspension culturet0
Outcome
ammonium ione0
Direction
most rapidlye0
Comparator
five cationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice cell suspension culture”

✓ located verbatim in the source · title, characters 71–99 · show in text ↑
e0

“ammonium ion, which, of five cations, was taken up most rapidly”

✓ located verbatim in the source · abstract, characters 254–317 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Malic acid and some citric acid were the principal iron-solubilizing compounds released by cultured rice cells.

Organ / stage
cultured rice cellse0
Outcome
malic acid and some citric acide0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“These iron-solubilizing compounds from cultured rice cells were identified by chromatography to be principally malic acid and some citric acid.”

✓ located verbatim in the source · abstract, characters 319–462 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

The growth rate of rice cells declined at low inoculum density in medium containing nearly insoluble ferric chloride.

Treatment
low population densitye0
Outcome
growth rate of the cultured cellse0
Direction
decreasede0
Qualifier
almost insoluble ferric chloride as the iron sourcee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice cell suspension culture”

✓ located verbatim in the source · title, characters 71–99 · show in text ↑
e0

“In the medium containing almost insoluble ferric chloride as the iron source, the growth rate of the cultured cells decreased when cells were inoculated at a low population density.”

✓ located verbatim in the source · abstract, characters 463–644 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding conditioned medium rectified iron deficiency at low initial cell population.

Treatment
addition of a small amount of conditioned mediume0
Outcome
iron deficiencye0
Direction
rectifiede0
Qualifier
low initial populatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice cell suspension culture”

✓ located verbatim in the source · title, characters 71–99 · show in text ↑
e0

“This iron deficiency at the low initial population was rectified by an addition of a small amount of conditioned medium or malic acid.”

✓ located verbatim in the source · abstract, characters 645–779 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding malic acid rectified iron deficiency at low initial cell population.

Treatment
malic acide0
Outcome
iron deficiencye0
Direction
rectifiede0
Qualifier
low initial populatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice cell suspension culture”

✓ located verbatim in the source · title, characters 71–99 · show in text ↑
e0

“This iron deficiency at the low initial population was rectified by an addition of a small amount of conditioned medium or malic acid.”

✓ located verbatim in the source · abstract, characters 645–779 · show in text ↑

malic acid · nutrient: content · Oryza sativa (rice)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

in vitro · nutrient: content · Oryza sativa (rice)Positive and negative results

11 papers: 9 positive, 2 negative or no effect

8 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.