Paper · 2009 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Chromium-induced accumulation of peroxide content, stimulation of antioxidative enzymes and lipid peroxidation in green gram (Vigna radiata L. cv. Wilczek) leaves

Pritam Bala Sinha; Kamarul Haniya A; Kumariah Manoharan

6
claims
12
verbatim quotes
15
citations
1
research areas
✓
Open access

Abstract

Chromium (Cr)-induced oxidative damage and changes in contents of chlorophyll, protein, peroxide and malondialdehyde (MDA) and activities of enzymatic antioxidants were investigated in 4-day-old green gram (Vigna radiata L. cv. Wilczek) seedlings. Cr increased the contents of peroxide and MDA but decreased the contents of chlorophyll and proteins. Cr reduced the activities of catalase (CAT) and glutathione reductase (GR), but increased the activity of superoxide dismutase (SOD) in green gram leaves. Lipid peroxidation is considered to be an important mechanism of Cr-induced oxidative damage in green gram leaves. The peroxidation of lipids can be initiated by free radicals. The effects of Crinduced oxidative damage and increase in the contents of peroxide and MDA in green gram leaves can be minimized by pretreatment with ascorbic acid (AA) and reduced glutathione (GSH) or both.

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chromium increased peroxide and MDA contents in green gram leaves.

Material
cv. Wilczekt0
Organ / stage
leavest0
Treatment
Cre1
Outcome
contents of peroxide and MDAe1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e1

“Cr increased the contents of peroxide and MDA but decreased the contents of chlorophyll and proteins”

✓ located verbatim in the source · abstract, characters 248–348 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chromium decreased chlorophyll and protein contents in green gram leaves.

Material
cv. Wilczekt0
Organ / stage
leavest0
Treatment
Cre1
Outcome
contents of chlorophyll and proteinse1
Direction
decreasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e1

“Cr increased the contents of peroxide and MDA but decreased the contents of chlorophyll and proteins”

✓ located verbatim in the source · abstract, characters 248–348 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chromium reduced catalase and glutathione reductase activities in green gram leaves.

Material
cv. Wilczekt0
Organ / stage
leavest0
Treatment
Cre2
Outcome
activities of catalase (CAT) and glutathione reductase (GR)e2
Direction
reducede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e2

“Cr reduced the activities of catalase (CAT) and glutathione reductase (GR), but increased the activity of superoxide dismutase (SOD) in green gram leaves”

✓ located verbatim in the source · abstract, characters 350–503 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Chromium increased superoxide dismutase activity in green gram leaves.

Material
cv. Wilczekt0
Organ / stage
leavest0
Treatment
Cre2
Outcome
activity of superoxide dismutase (SOD)e2
Direction
increasede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e2

“Cr reduced the activities of catalase (CAT) and glutathione reductase (GR), but increased the activity of superoxide dismutase (SOD) in green gram leaves”

✓ located verbatim in the source · abstract, characters 350–503 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Lipid peroxidation is considered an important mechanism of Cr-induced oxidative damage in green gram leaves.

Outcome
oxidative damagee3
Qualifier
Lipid peroxidatione3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e3

“Lipid peroxidation is considered to be an important mechanism of Cr-induced oxidative damage in green gram leaves”

✓ located verbatim in the source · abstract, characters 505–618 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pretreatment with ascorbic acid and reduced glutathione, alone or together, can minimize Cr-induced oxidative damage and the increase in peroxide and MDA in green gram leaves.

Treatment
pretreatment with ascorbic acid (AA) and reduced glutathione (GSH) or bothe4
Outcome
effects of Crinduced oxidative damagee4
Direction
minimizede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“green gram (Vigna radiata L. cv. Wilczek) leaves”

✓ located verbatim in the source · title, characters 114–162 · show in text ↑
e4

“The effects of Crinduced oxidative damage and increase in the contents of peroxide and MDA in green gram leaves can be minimized by pretreatment with ascorbic acid (AA) and reduced glutathione (GSH) or both”

✓ located verbatim in the source · abstract, characters 682–888 · show in text ↑

chromium atom · physiology: antioxidant enzymes · Vigna radiata (mung bean)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative
See all 4 papers →

chromium atom · physiology: pigments · Vigna radiata (mung bean)Positive and negative results

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

glutathione · physiology: oxidative · Vigna radiata (mung bean)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

chromium atom · physiology: oxidative · Vigna radiata (mung bean)Results agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.