Abstract
A light-induced chloroplast shrinkage occurring in vivo was measured with a Coulter counter and a packed weight technique using chloroplasts isolated within two minutes of harvesting pea plants. Introduction of the photosynthetic inhibitor DCMU (5 μM) into the plant either by bathing the cut stem or injection through a fine hypodermic needle decreased the light-induced chloroplast shrinkage in vivo 11 to 20%. The uncoupler tri-Fl-CCP (5 μM) inhibited the light-induced shrinkage 80 % , while nigericin (0.5 μM) completely abolished it. An action spectrum for the chloroplast volume decrease in vivo had a shoulder at 700-715 mμ. These results are considered in terms of the various forms of energy available during photosynthesis. A consistent interpretation is that the lightinduced chloroplast shrinkage in vivo depends either on a high energy state created by electron flow or on ATP. This chloroplast volume change upon illumination of the plants may increase the photosynthetic efficiency.
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Claims 6
Claim · model-written summary
DCMU decreased light-induced chloroplast shrinkage by 11 to 20%.
- Taxon
- pea plants
Lathyrus oleraceuse1 - Treatment
- DCMU (5 μM)e0
- Outcome
- light-induced chloroplast shrinkagee0
- Direction
- decreasede0
- Value
- 11 to 20%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DCMU (5 μM) into the plant either by bathing the cut stem or injection through a fine hypodermic needle decreased the light-induced chloroplast shrinkage in vivo 11 to 20%”
e1“using chloroplasts isolated within two minutes of harvesting pea plants”
Claim · model-written summary
Tri-Fl-CCP inhibited light-induced chloroplast shrinkage by 80%.
- Taxon
- pea plantsLathyrus oleraceuse1
- Treatment
- tri-Fl-CCP (5 μM)e0
- Outcome
- light-induced shrinkagee0
- Direction
- inhibitede0
- Value
- 80 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The uncoupler tri-Fl-CCP (5 μM) inhibited the light-induced shrinkage 80 %”
t0“chloroplast shrinkage in vivo”
e1“using chloroplasts isolated within two minutes of harvesting pea plants”
Claim · model-written summary
Nigericin completely abolished light-induced chloroplast shrinkage.
- Taxon
- pea plantsLathyrus oleraceuse1
- Treatment
- nigericin (0.5 μM)e0
- Outcome
- light-induced chloroplast shrinkagee2
- Direction
- completely abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nigericin (0.5 μM) completely abolished it”
e1“using chloroplasts isolated within two minutes of harvesting pea plants”
e2“DCMU (5 μM) into the plant either by bathing the cut stem or injection through a fine hypodermic needle decreased the light-induced chloroplast shrinkage in vivo 11 to 20%”
Claim · model-written summary
The action spectrum for chloroplast volume decrease had a shoulder at 700-715 mμ.
- Taxon
- pea plantsLathyrus oleraceuse1
- Outcome
- action spectrum for the chloroplast volume decreasee0
- Value
- 700-715 mμe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An action spectrum for the chloroplast volume decrease in vivo had a shoulder at 700-715 mμ”
e1“using chloroplasts isolated within two minutes of harvesting pea plants”
Claim · model-written summary
The authors interpret chloroplast shrinkage as dependent on a high energy state or ATP.
- Taxon
- pea plantsLathyrus oleraceuse1
- Outcome
- lightinduced chloroplast shrinkagee0
- Qualifier
- a high energy state created by electron flow or on ATPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lightinduced chloroplast shrinkage in vivo depends either on a high energy state created by electron flow or on ATP”
e1“using chloroplasts isolated within two minutes of harvesting pea plants”
Claim · model-written summary
The authors suggest illumination-driven chloroplast volume change may increase photosynthetic efficiency.
- Taxon
- plantsViridiplantaee0
- Treatment
- illuminatione0
- Outcome
- photosynthetic efficiencye0
Verbatim
e0“This chloroplast volume change upon illumination of the plants may increase the photosynthetic efficiency”