Abstract
A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank. The premature senescence phenotype appeared at the six-leaf stage and the plant died at the early heading stage. psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content. Furthermore, the expression of senescence-related genes was significantly altered in psd128. The mutant trait was controlled by a single recessive nuclear gene. Using map-based strategy, the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA-type ATPase with 809 amino-acid residuals. A single base substitution at position C2347T in psd128 resulted in a premature stop codon. Functional complementation could rescue the mutant phenotype. In addition, RNA interference resulted in the premature senescence and death phenotype. OsCDC48 was expressed constitutively in the root, stem, leaf and panicle. Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus. OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species. Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.
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Claims 15
Claim · model-written summary
The psd128 mutant shows reduced photosynthetic ability, chlorophyll and carotenoid contents.
- Taxon
- rice
Oryza sativae0 - Material
- psd128e2
- Outcome
- photosynthetic ability, chlorophyll and carotenoid contentse2
- Direction
- reducede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e2“psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content”
Claim · model-written summary
The psd128 mutant shows reduced root vigor and soluble protein content.
- Taxon
- riceOryza sativae0
- Material
- psd128e2
- Outcome
- root vigor, soluble protein contente2
- Direction
- reducede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e2“psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content”
Claim · model-written summary
The psd128 mutant shows increased malonaldehyde content.
- Taxon
- riceOryza sativae0
- Material
- psd128e2
- Outcome
- malonaldehyde contente2
- Direction
- increasede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e2“psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content”
Claim · model-written summary
Expression of senescence-related genes is significantly altered in psd128.
- Taxon
- riceOryza sativae0
- Material
- psd128e3
- Outcome
- expression of senescence-related genese3
- Direction
- alterede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e3“Furthermore, the expression of senescence-related genes was significantly altered in psd128”
Claim · model-written summary
The mutant trait is controlled by a single recessive nuclear gene.
- Taxon
- riceOryza sativae0
- Outcome
- mutant traite4
- Value
- single recessive nuclear genee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e4“The mutant trait was controlled by a single recessive nuclear gene”
Claim · model-written summary
A single base substitution at position C2347T in OsCDC48 of psd128 results in a premature stop codon.
- Taxon
- riceOryza sativae0
- Material
- psd128e6
- Treatment
- single base substitution at position C2347Te6
- Gene
- OsCDC48e5
- Outcome
- premature stop codone6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e5“the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA-type ATPase with 809 amino-acid residuals”
e6“A single base substitution at position C2347T in psd128 resulted in a premature stop codon”
Claim · model-written summary
Functional complementation rescues the mutant phenotype.
- Taxon
- riceOryza sativae0
- Treatment
- Functional complementatione7
- Outcome
- mutant phenotypee7
- Direction
- rescuee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e7“Functional complementation could rescue the mutant phenotype”
Claim · model-written summary
RNA interference results in the premature senescence and death phenotype.
- Taxon
- riceOryza sativae0
- Treatment
- RNA interferencee7
- Outcome
- premature senescence and death phenotypee7
- Direction
- resulted ine7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e7“RNA interference resulted in the premature senescence and death phenotype”
Claim · model-written summary
OsCDC48 is expressed constitutively in the root, stem, leaf and panicle.
- Taxon
- riceOryza sativae0
- Organ / stage
- root, stem, leaf and paniclee7
- Gene
- OsCDC48e7
- Outcome
- expressed constitutivelye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e7“OsCDC48 was expressed constitutively in the root, stem, leaf and panicle”
Claim · model-written summary
OsCDC48:YFP fusion proteins are located both in the cytoplasm and nucleus.
- Taxon
- riceOryza sativae0
- Gene
- OsCDC48:YFP fusion proteinse7
- Outcome
- located both in the cytoplasm and nucleuse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e7“OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus”
Claim · model-written summary
OsCDC48 is highly conserved, with more than 90% protein identity among plant species.
Verbatim
e7“OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species”
Claim · model-written summary
The impaired function of OsCDC48 is responsible for the premature senescence and death phenotype.
- Taxon
- riceOryza sativae0
- Gene
- OsCDC48e7
- Outcome
- premature senescence and death phenotypee7
- Qualifier
- impaired functione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e7“Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype”
Claim · model-written summary
The premature senescence phenotype appears at the six-leaf stage.
- Taxon
- riceOryza sativae0
- Organ / stage
- six-leaf stagee1
- Outcome
- premature senescence phenotype appearede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e1“The premature senescence phenotype appeared at the six-leaf stage and the plant died at the early heading stage”
Claim · model-written summary
The plant died at the early heading stage.
- Taxon
- riceOryza sativae0
- Organ / stage
- early heading stagee1
- Outcome
- plant diede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e1“The premature senescence phenotype appeared at the six-leaf stage and the plant died at the early heading stage”
Claim · model-written summary
OsCDC48 is predicted to encode a putative AAA-type ATPase with 809 amino-acid residues.
- Taxon
- riceOryza sativae0
- Gene
- OsCDC48e5
- Outcome
- predicted to encode a putative AAA-type ATPasee5
- Value
- 809 amino-acid residualse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank”
e5“the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA-type ATPase with 809 amino-acid residuals”
The same questions in other papers
loss of function · quality: postharvest · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 6 in the opposite direction
- positive
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9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.