Abstract
Lipid peroxidation plays a major role in seed longevity and viability. In rice grains, lipid peroxidation is catalyzed by the enzyme lipoxygenase 3 (LOX3). Previous reports showed that grain from the rice variety DawDam in which the LOX3 gene was deleted had less stale flavour after grain storage than normal rice. The molecular mechanism by which LOX3 expression is regulated during endosperm development remains unclear. In this study, we expressed a LOX3 antisense construct in transgenic rice (Oryza sativa L.) plants to down-regulate LOX3 expression in rice endosperm. The transgenic plants exhibited a marked decrease in LOX mRNA levels, normal phenotypes and a normal life cycle. We showed that LOX3 activity and its ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD) from linoleic acid were significantly lower in transgenic seeds than in wild-type seeds by measuring the ultraviolet absorption of 9-HPOD at 234 nm and by high-performance liquid chromatography. The suppression of LOX3 expression in rice endosperm increased grain storability. The germination rate of TS-91 (antisense LOX3 transgenic line) was much higher than the WT (29% higher after artificial ageing for 21 days, and 40% higher after natural ageing for 12 months). To our knowledge, this is the first report to demonstrate that decreased LOX3 expression can preserve rice grain quality during storage with no impact on grain yield, suggesting potential applications in agricultural production.
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Claims 9
Claim · model-written summary
LOX3 antisense transgenic rice had markedly lower LOX mRNA.
- Taxon
- rice (Oryza sativa L.)
Oryza sativae1 - Treatment
- LOX3 antisense constructe2
- Gene
- LOXe0
- Outcome
- LOX mRNA levelse0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants exhibited a marked decrease in LOX mRNA levels”
e1“transgenic rice (Oryza sativa L.) plants”
e2“LOX3 antisense construct”
Claim · model-written summary
LOX3 antisense transgenic rice had normal phenotypes and life cycle.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Outcome
- normal phenotypes and a normal life cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants exhibited a marked decrease in LOX mRNA levels, normal phenotypes and a normal life cycle.”
e1“transgenic rice (Oryza sativa L.) plants”
Claim · model-written summary
LOX3 activity was significantly lower in transgenic seeds than wild type.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Treatment
- LOX3 antisense constructe2
- Gene
- LOX3e0
- Outcome
- LOX3 activitye0
- Direction
- lowere0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LOX3 activity and its ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD) from linoleic acid were significantly lower in transgenic seeds than in wild-type seeds”
e1“transgenic rice (Oryza sativa L.) plants”
e2“LOX3 antisense construct”
Claim · model-written summary
LOX3 ability to produce 9-HPOD from linoleic acid was significantly lower in transgenic seeds.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Treatment
- LOX3 antisense constructe2
- Gene
- LOX3e0
- Outcome
- ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD)e0
- Direction
- lowere0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LOX3 activity and its ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD) from linoleic acid were significantly lower in transgenic seeds than in wild-type seeds”
e1“transgenic rice (Oryza sativa L.) plants”
e2“LOX3 antisense construct”
Claim · model-written summary
LOX3 suppression increased rice grain storability.
- Taxon
- riceOryza sativae0
- Treatment
- suppression of LOX3 expressione0
- Gene
- LOX3e0
- Outcome
- grain storabilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of LOX3 expression in rice endosperm increased grain storability.”
Claim · model-written summary
TS-91 germination was 29% higher than wild type after 21 days of artificial ageing.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- TS-91e0
- Treatment
- artificial ageing for 21 dayse0
- Outcome
- germination ratee0
- Direction
- highere0
- Value
- 29%e0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination rate of TS-91 (antisense LOX3 transgenic line) was much higher than the WT (29% higher after artificial ageing for 21 days, and 40% higher after natural ageing for 12 months).”
e1“transgenic rice (Oryza sativa L.) plants”
Claim · model-written summary
TS-91 germination was 40% higher than wild type after 12 months of natural ageing.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- TS-91e0
- Treatment
- natural ageing for 12 monthse0
- Outcome
- germination ratee0
- Direction
- highere0
- Value
- 40%e0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination rate of TS-91 (antisense LOX3 transgenic line) was much higher than the WT (29% higher after artificial ageing for 21 days, and 40% higher after natural ageing for 12 months).”
e1“transgenic rice (Oryza sativa L.) plants”
Claim · model-written summary
Decreased LOX3 expression preserved rice grain quality during storage.
- Taxon
- riceOryza sativae0
- Treatment
- decreased LOX3 expressione0
- Gene
- LOX3e0
- Outcome
- rice grain qualitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased LOX3 expression can preserve rice grain quality during storage with no impact on grain yield”
Claim · model-written summary
Decreased LOX3 expression had no impact on rice grain yield.
- Taxon
- riceOryza sativae1
- Treatment
- decreased LOX3 expressione0
- Outcome
- grain yielde0
- Direction
- no impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased LOX3 expression can preserve rice grain quality during storage with no impact on grain yield”
e1“preserve rice grain quality during storage”
The same questions in other papers
loss of function · quality: postharvest · Oryza sativa (rice)Positive and negative results
5 papers in the same direction as this paper · 7 in the opposite direction
- positive
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.