Paper · 2014 · Molecular Biology

RNA silencing of the anionic peroxidase gene impairs potato plant resistance to Phytophthora infestans (Mont.) de Bary

A. V. Sorokan; B. R. Kuluev; G. F. Burkhanova; et al.

4
claims
9
verbatim quotes
10
citations
3
research areas
—
Not open access

Abstract

Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment were obtained by agrobacterial transformation. A manifold decrease in activity of anionic isoperoxidase with pI ∼ 3.5 in the transformed plants demonstrated that the enzyme is encoded by M21334 . The transformed plants showed a decrease in lignin accumulation and a dramatically lower resistance to the late blight agent Phytophthora infestans , implicating the enzyme in the response to P. infestans infection.

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Claims 4

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Antisense M21334 potato plants had markedly lower anionic isoperoxidase activity.

Treatment
antisense M21334 fragmente0
Gene
M21334e0
Outcome
activity of anionic isoperoxidasee1
Direction
decreasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment”

✓ located verbatim in the source · abstract, characters 0–74 · show in text ↑
e1

“A manifold decrease in activity of anionic isoperoxidase with pI ∼ 3.5 in the transformed plants”

✓ located verbatim in the source · abstract, characters 122–218 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The lower enzyme activity indicates that M21334 encodes the anionic isoperoxidase.

Gene
M21334e0
Outcome
anionic isoperoxidasee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the enzyme is encoded by M21334”

✓ located verbatim in the source · abstract, characters 237–268 · show in text ↑
e1

“anionic isoperoxidase with pI ∼ 3.5”

✓ located verbatim in the source · abstract, characters 157–192 · show in text ↑
e2

“Potato Solanum tuberosum L. plants”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Transformed potato plants accumulated less lignin.

Treatment
antisense M21334 fragmente1
Outcome
lignin accumulatione0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The transformed plants showed a decrease in lignin accumulation”

✓ located verbatim in the source · abstract, characters 271–334 · show in text ↑
e1

“Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment”

✓ located verbatim in the source · abstract, characters 0–74 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Transformed potato plants had dramatically lower resistance to Phytophthora infestans.

Treatment
antisense M21334 fragmente1
Interactor
Phytophthora infestansPhytophthora infestanse0pathogen
Outcome
resistance to the late blight agent Phytophthora infestanse0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗
Phytophthora infestans
Phytophthora infestans · InteractorHoward F. Schwartz, Colorado State University, United States · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The transformed plants showed a decrease in lignin accumulation and a dramatically lower resistance to the late blight agent Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 271–418 · show in text ↑
e1

“Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment”

✓ located verbatim in the source · abstract, characters 0–74 · show in text ↑

loss of function · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results

22 papers: 9 positive, 13 negative or no effect

12 papers in the same direction as this paper · 9 in the opposite direction

  1. negative
  2. positive
  3. positive

18 papers more in this group

See all 22 papers →

loss of function · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results

31 papers: 16 positive, 15 negative or no effect

14 papers in the same direction as this paper · 16 in the opposite direction

  1. negative
  2. positive
  3. positive

27 papers more in this group

See all 31 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.