Abstract
Fructan synthesis was induced in excised primary leaf blades of Hordeum vulgare L. cv Gerbel by illumination in 30 millimolar fructose. This treatment induced a 26-fold increase of sucrose-sucrose-fructosyltransferase (SST, EC 2.4.1.99) activity within 24 hours. Acid invertase (EC 3.2.1.26) activity remained about constant. By preparing protoplasts from induced leaves, approximately 80% of the invertase activity was removed with the cell walls while SST was retained. The protoplast homogenate was used to partially purify and characterize SST. Acid precipitation (pH 4.75) and anion exchange chromatography (fast protein liquid chromatography on Mono ;Q') resulted in a recovery of about 80% of total SST activity. The principal activity (SST 1), accounting for 85% of the activity recovered, was purified about 200-fold. It was essentially free of invertase activity and catalyzed the synthesis of a trisaccharide which co-chromatographed with isokestose (1F-beta-fructosylsucrose). The remaining 15% of SST activity (SST 2) was purified about 35-fold. It retained substantial invertase activity and catalyzed the synthesis of only one trisaccharide which co-chromatographed with kestose (6F-beta-fructosylsucrose). It is concluded that barley leaves which store mainly fructan of the phlein type (beta-2-6 polyfructosylsucrose), nevertheless contain sucrose-sucrose 1F-beta-d-fructosyltransferase as the key enzyme of fructan synthesis.
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Claims 13
Claim · model-written summary
Illumination in fructose induced fructan synthesis in excised barley leaves.
- Taxon
- Hordeum vulgare L.
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Hordeum vulgaree0 - Material
- cv Gerbele0
- Organ / stage
- primary leaf bladese0
- Treatment
- illumination in 30 millimolar fructosee0
- Outcome
- Fructan synthesise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Fructan synthesis was induced in excised primary leaf blades of Hordeum vulgare L. cv Gerbel by illumination in 30 millimolar fructose”
Claim · model-written summary
The treatment increased SST activity 26-fold within 24 hours.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Treatment
- This treatmente0
- Gene
- sucrose-sucrose-fructosyltransferasee0
- Outcome
- sucrose-sucrose-fructosyltransferase (SST, EC 2.4.1.99) activitye0
- Direction
- increasee0
- Value
- 26-folde0
- Qualifier
- within 24 hourse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This treatment induced a 26-fold increase of sucrose-sucrose-fructosyltransferase (SST, EC 2.4.1.99) activity within 24 hours”
e1“Hordeum vulgare L.”
Claim · model-written summary
Acid invertase activity remained about constant after treatment.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- Acid invertasee0
- Outcome
- Acid invertase (EC 3.2.1.26) activitye0
- Direction
- remained about constante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acid invertase (EC 3.2.1.26) activity remained about constant”
e1“Hordeum vulgare L.”
Claim · model-written summary
Protoplast preparation removed approximately 80% of invertase activity with cell walls while retaining SST.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Outcome
- invertase activitye0
- Direction
- removede0
- Value
- approximately 80%e0
- Qualifier
- with the cell walls while SST was retainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“approximately 80% of the invertase activity was removed with the cell walls while SST was retained”
e1“Hordeum vulgare L.”
Claim · model-written summary
SST purification recovered about 80% of total activity.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- SSTe0
- Outcome
- recovery of about 80% of total SST activitye0
- Value
- about 80%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acid precipitation (pH 4.75) and anion exchange chromatography (fast protein liquid chromatography on Mono ;Q') resulted in a recovery of about 80% of total SST activity”
e1“Hordeum vulgare L.”
Claim · model-written summary
SST 1 accounted for 85% of recovered activity.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- SST 1e0
- Outcome
- activity recoverede0
- Value
- 85%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The principal activity (SST 1), accounting for 85% of the activity recovered”
e1“Hordeum vulgare L.”
Claim · model-written summary
SST 1 was purified about 200-fold.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- SST 1e0
- Outcome
- purifiede0
- Value
- about 200-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SST 1), accounting for 85% of the activity recovered, was purified about 200-fold”
e1“Hordeum vulgare L.”
Claim · model-written summary
SST 1 was essentially free of invertase activity.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree2
- Gene
- SST 1e1
- Outcome
- essentially free of invertase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was essentially free of invertase activity”
e1“SST 1”
e2“Hordeum vulgare L.”
Claim · model-written summary
SST 1 catalyzed a trisaccharide that co-chromatographed with isokestose.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree2
- Gene
- SST 1e1
- Outcome
- trisaccharide which co-chromatographed with isokestosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catalyzed the synthesis of a trisaccharide which co-chromatographed with isokestose (1F-beta-fructosylsucrose)”
e1“SST 1”
e2“Hordeum vulgare L.”
Claim · model-written summary
SST 2 comprised the remaining 15% of activity and was purified about 35-fold.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- SST 2e0
- Outcome
- purifiede0
- Value
- about 35-folde0
- Qualifier
- remaining 15% of SST activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The remaining 15% of SST activity (SST 2) was purified about 35-fold”
e1“Hordeum vulgare L.”
Claim · model-written summary
SST 2 retained substantial invertase activity.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree2
- Gene
- SST 2e1
- Outcome
- substantial invertase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It retained substantial invertase activity”
e1“SST 2”
e2“Hordeum vulgare L.”
Claim · model-written summary
SST 2 catalyzed one trisaccharide co-chromatographing with kestose.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree2
- Gene
- SST 2e1
- Outcome
- only one trisaccharide which co-chromatographed with kestosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catalyzed the synthesis of only one trisaccharide which co-chromatographed with kestose (6F-beta-fructosylsucrose)”
e1“SST 2”
e2“Hordeum vulgare L.”
Claim · model-written summary
Barley leaves were concluded to contain sucrose-sucrose 1F-beta-d-fructosyltransferase as a key enzyme of fructan synthesis.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- leavese0
- Gene
- sucrose-sucrose 1F-beta-d-fructosyltransferasee0
- Outcome
- fructan synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley leaves which store mainly fructan of the phlein type (beta-2-6 polyfructosylsucrose), nevertheless contain sucrose-sucrose 1F-beta-d-fructosyltransferase as the key enzyme of fructan synthesis”
The same questions in other papers
light · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
18 papers in the same direction as this paper · 11 in the opposite direction
- no effect
- negative
- negative
26 papers more in this group
See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.