Abstract
Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line and from healthy and rust-infected wheat leaves of a resistant line. Extracts from rust-infected leaves of both lines contained 50% more folates, active as growth factors for Lactobacillus casei, than did those from healthy leaves. Rust-infected leaves did not differ from healthy leaves in their content of folates active as growth factors for Streptococcus faecalis and Pediococcus cerevisiae.All extracts were fractionated on DEAE-cellulose columns. The eluate fractions were treated with chicken pancreas conjugase and assayed with L. casei. All extracts yielded similar folate profiles consisting of five major and three minor peaks. Conjugase treatment and differential assay of the peak fractions with L. casei, S. faecalis, and P. cerevisiae indicated that the eluates contained folates methylated at N-5 and folates formylated at N-10 in various states of oxidation and conjugation. The eluates also contained a compound believed to be 5-formyltetrahydropteroylglutamate, small amounts of pteroylglutamate, traces of tetrahydropteroylglutamate, and several unidentified folates.The increase of folate levels in rust-infected leaves was due almost entirely to increases of 5-methyl-tetrahydropteroylgiutamate and its conjugates. The folate composition of resistant-reacting leaves did not differ appreciably from that of susceptible-reacting leaves.Radioactivity was not incorporated into either 5-methyltetrahydropteroyltriglutamate or into methionine when 5-[methyl- 14 C]-tetrahydropteroylglutamate was fed to wheat leaves.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Rust-infected leaves of both lines contained 50% more folates active for Lactobacillus casei than healthy leaves.
- Taxon
- wheat
Triticum aestivume0 - Material
- susceptible linee0
- Organ / stage
- leavese1
- Treatment
- rust-infectede1
- Interactor
- Ruste1pathogen
- Outcome
- folatese1
- Direction
- moree1
- Value
- 50%e1
- Comparator
- healthy leavese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line”
e1“Extracts from rust-infected leaves of both lines contained 50% more folates, active as growth factors for Lactobacillus casei, than did those from healthy leaves”
Claim · model-written summary
Rust-infected and healthy wheat leaves did not differ in folates active for Streptococcus faecalis and Pediococcus cerevisiae.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Rust-infectede2
- Interactor
- Ruste2pathogen
- Outcome
- content of folatese2
- Direction
- did not differe2
- Comparator
- healthy leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line”
e2“Rust-infected leaves did not differ from healthy leaves in their content of folates active as growth factors for Streptococcus faecalis and Pediococcus cerevisiae”
Claim · model-written summary
The increase of folate levels in rust-infected wheat leaves was due almost entirely to increases of 5-methyl-tetrahydropteroylglutamate and its conjugates.
- Taxon
- wheatTriticum aestivume0
- Treatment
- rust-infectede3
- Compound
- 5-methyl-tetrahydropteroylgiutamatee3
- Outcome
- folate levelse3
- Direction
- increasee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line”
e3“The increase of folate levels in rust-infected leaves was due almost entirely to increases of 5-methyl-tetrahydropteroylgiutamate and its conjugates”
Claim · model-written summary
Radioactivity from 5-[methyl- 14 C]-tetrahydropteroylglutamate was not incorporated into 5-methyltetrahydropteroyltriglutamate or methionine in wheat leaves.
- Taxon
- wheatTriticum aestivume4
- Treatment
- 5-[methyl- 14 C]-tetrahydropteroylglutamatee4
- Outcome
- Radioactivity was not incorporated into either 5-methyltetrahydropteroyltriglutamate or into methioninee4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Radioactivity was not incorporated into either 5-methyltetrahydropteroyltriglutamate or into methionine when 5-[methyl- 14 C]-tetrahydropteroylglutamate was fed to wheat leaves”
Claim · model-written summary
All wheat leaf extracts yielded similar folate profiles of five major and three minor peaks.
- Taxon
- wheatTriticum aestivume0
- Outcome
- folate profilese5
- Qualifier
- five major and three minor peakse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line”
e5“All extracts yielded similar folate profiles consisting of five major and three minor peaks”
Claim · model-written summary
The folate composition of resistant-reacting wheat leaves did not differ appreciably from that of susceptible-reacting leaves.
- Taxon
- wheatTriticum aestivume0
- Material
- resistant-reacting leavese6
- Outcome
- folate compositione6
- Direction
- did not differ appreciablye6
- Comparator
- susceptible-reacting leavese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six days after inoculation, crude extracts were prepared from healthy and rust-infected wheat leaves of a susceptible line”
e6“The folate composition of resistant-reacting leaves did not differ appreciably from that of susceptible-reacting leaves”
The same questions in other papers
methyl · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.