Paper · 1988 · Journal of the Science of Food and Agriculture

Determination of total nitrogen and cyanide nitrogen in cassava

Elinor Torres; José F. Pereira; Anita M. Brinker; et al.

7
claims
8
verbatim quotes
8
citations
1
research areas
—
Not open access

Abstract

Abstract Heating of intact cassava leaves causes liberation of hydrogen cyanide. This phenomenon appears to be caused by β‐glucosidase‐catalysed decomposition of the cyanogenic glycosides linamarin and lotaustralin. Because of these losses, many previously determined values for total nitrogen in cassava (and other cyanogenic plants) are probably in error as plant materials used for Kjeldahl determinations are often dried at 70–90°C. Liberation of cyanide from intact cassava leaves by heating at 80°C is more complete than liberation by the homogenisation methods commonly used. Homogenisation of cassava leaf tissues with liquid nitrogen or dry ice gives somewhat lower values, and these materials are often not readily available in areas where cassava is studied. The basis of a relatively simple procedure for the isolation of hydrogen cyanide from cassava leaves is suggested.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Heating intact cassava leaves liberates hydrogen cyanide.

Organ / stage
leavese0
Treatment
Heatinge0
Outcome
liberation of hydrogen cyanidee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Heating of intact cassava leaves causes liberation of hydrogen cyanide.”

✓ located verbatim in the source · abstract, characters 9–80 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors attribute cyanide liberation to glycoside decomposition catalysed by β-glucosidase.

Gene
β‐glucosidasee0
Compound
linamarin and lotaustraline0
Outcome
decomposition of the cyanogenic glycosides linamarin and lotaustraline0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“This phenomenon appears to be caused by β‐glucosidase‐catalysed decomposition of the cyanogenic glycosides linamarin and lotaustralin.”

✓ located verbatim in the source · abstract, characters 81–215 · show in text ↑
t0

“cassava”

✓ located verbatim in the source · title, characters 56–63 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Previously measured total nitrogen values in cassava are probably erroneous because drying can lose cyanide nitrogen.

Treatment
dried at 70–90°Ce0
Outcome
values for total nitrogene0
Qualifier
probably in errore0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Because of these losses, many previously determined values for total nitrogen in cassava (and other cyanogenic plants) are probably in error as plant materials used for Kjeldahl determinations are often dried at 70–90°C.”

✓ located verbatim in the source · abstract, characters 216–436 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Heating intact cassava leaves at 80°C releases cyanide more completely than common homogenisation methods.

Treatment
heating at 80°Ce0
Outcome
Liberation of cyanidee0
Direction
more completee0
Comparator
homogenisation methods commonly usede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Liberation of cyanide from intact cassava leaves by heating at 80°C is more complete than liberation by the homogenisation methods commonly used.”

✓ located verbatim in the source · abstract, characters 437–582 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Homogenisation with liquid nitrogen yields somewhat lower values.

Treatment
liquid nitrogene0
Outcome
valuese0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Homogenisation of cassava leaf tissues with liquid nitrogen or dry ice gives somewhat lower values”

✓ located verbatim in the source · abstract, characters 583–681 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Homogenisation with dry ice yields somewhat lower values.

Treatment
dry icee0
Outcome
valuese0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Homogenisation of cassava leaf tissues with liquid nitrogen or dry ice gives somewhat lower values”

✓ located verbatim in the source · abstract, characters 583–681 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest a relatively simple procedure for isolating hydrogen cyanide from cassava leaves.

Outcome
procedure for the isolation of hydrogen cyanidee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“The basis of a relatively simple procedure for the isolation of hydrogen cyanide from cassava leaves is suggested.”

✓ located verbatim in the source · abstract, characters 770–884 · show in text ↑

heat · molecular: metabolites · Manihot esculenta (cassava)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.