Abstract
Contrasting with the wealth of information available on the multiple roles of jasmonates in plant development and defense, knowledge about the functions and the biosynthesis of hydroxylated oxylipins remains scarce. By expressing the caleosin RESPONSIVE TO DESSICATION20 (RD20) in Saccharomyces cerevisiae, we show that the recombinant protein possesses an unusual peroxygenase activity with restricted specificity toward hydroperoxides of unsaturated fatty acid. Accordingly, Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid, a linolenate-derived hydroxide. These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination. These phenotypes are dependent on the presence of active RD20, since they are abolished in the rd20 null mutant and in lines overexpressing RD20, in which peroxygenase was inactivated by a point mutation of a catalytic histidine residue. RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid. Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition. A model is discussed where the interplay between fatty acid hydroxides generated by RD20 and ROS is counteracted by ethylene during development in unstressed environments.
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Claims 15
Claim · model-written summary
Recombinant RD20 protein has peroxygenase activity toward hydroperoxides of unsaturated fatty acids.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- RD20e1
- Outcome
- peroxygenase activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e1“By expressing the caleosin RESPONSIVE TO DESSICATION20 (RD20) in Saccharomyces cerevisiae, we show that the recombinant protein possesses an unusual peroxygenase activity”
Claim · model-written summary
Arabidopsis plants overexpressing RD20 accumulate 13-hydroxy-9,11,15-octadecatrienoic acid.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- overexpressing RD20e2
- Gene
- RD20e2
- Outcome
- 13-hydroxy-9,11,15-octadecatrienoic acide2
- Direction
- accumulatee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
Claim · model-written summary
RD20-overexpressing plants show elevated ROS levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- overexpressing RD20e2
- Outcome
- levels of reactive oxygen species (ROS)e3
- Direction
- elevatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
e3“These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination”
Claim · model-written summary
RD20-overexpressing plants flower early, in a gibberellin-dependent manner.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- overexpressing RD20e2
- Outcome
- floweringe3
- Direction
- earlye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
e3“These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination”
Claim · model-written summary
RD20-overexpressing plants are hypersensitive to abscisic acid at seed germination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- overexpressing RD20e2
- Outcome
- seed germinatione3
- Direction
- hypersensitivitye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
e3“These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination”
Claim · model-written summary
The phenotypes are abolished in the rd20 null mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- rd20 null mutante4
- Gene
- RD20e4
- Outcome
- phenotypese4
- Direction
- abolishede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
e4“These phenotypes are dependent on the presence of active RD20, since they are abolished in the rd20 null mutant”
Claim · model-written summary
The phenotypes are abolished in lines with inactivated peroxygenase by a point mutation of a catalytic histidine.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- point mutation of a catalytic histidine residuee5
- Gene
- RD20e5
- Outcome
- phenotypese4
- Direction
- abolishede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid”
e4“These phenotypes are dependent on the presence of active RD20, since they are abolished in the rd20 null mutant”
e5“and in lines overexpressing RD20, in which peroxygenase was inactivated by a point mutation of a catalytic histidine residue”
Claim · model-written summary
RD20 confers tolerance to Paraquat-induced stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e6“RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
RD20 confers tolerance to Rose Bengal-induced stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e6“RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
RD20 confers tolerance to heavy metal stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e6“RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
RD20 confers tolerance to the synthetic auxin 1-naphthaleneacetic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 1-naphthaleneacetic acide6
- Gene
- RD20e6
- Outcome
- tolerancee6
- Direction
- conferse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e6“RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
RD20 confers tolerance to the synthetic auxin 2,4-dichlorophenoxyacetic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 2,4-dichlorophenoxyacetic acide6
- Gene
- RD20e6
- Outcome
- tolerancee6
- Direction
- conferse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e6“RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
Under oxidative stress, RD20-overexpressing lines have reduced ROS levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- oxidative stresse7
- Outcome
- ROS levelse7
- Direction
- reducede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e7“Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition”
Claim · model-written summary
Under oxidative stress, RD20-overexpressing lines show minor cell death.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- oxidative stresse7
- Outcome
- cell deathe7
- Direction
- minore7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e7“Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition”
Claim · model-written summary
Under oxidative stress, RD20-overexpressing lines show delayed floral transition.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- oxidative stresse7
- Outcome
- floral transitione7
- Direction
- delayede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20”
e7“Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition”
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heavy metal · disease · ArabidopsisResults agree
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.