Abstract
Isolation of gene transcripts from desiccated leaf tissues of the resurrection grass, Sporobolus stapfianus, resulted in the identification of a gene, SDG8i, encoding a Group 1 glycosyltransferase (UGT). Here, we examine the effects of introducing this gene, under control of the CaMV35S promoter, into the model plant Arabidopsis thaliana. Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance. We hypothesise that expression of SDG8i in Arabidopsis negatively affects the bioactivity of metabolite/s that mediate/s environmentally-induced repression of cell division and expansion, both during normal development and in response to stress. The phenotype of transgenic plants over-expressing SDG8i suggests modulation in activities of both growth- and stress-related hormones. Plants overexpressing the UGT show evidence of elevated auxin levels, with the enzyme acting downstream of ABA to reduce drought-induced senescence. Analysis of the in vitro activity of the UGT recombinant protein product demonstrates that SDG8i can glycosylate the synthetic strigolactone analogue GR24, evoking a link with strigolactone-related processes in vivo. The large improvements observed in survival of transgenic Arabidopsis plants under cold-, salt- and drought-stress, as well as the substantial increases in growth rate and seed yield under non-stress conditions, indicates that overexpression of SDG8i in crop plants may provide a novel means of increasing plant productivity.
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Claims 9
Claim · model-written summary
Over-expression of SDG8i enhanced growth of Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise2 - Treatment
- constitutively over-expressing SDG8ie2
- Gene
- SDG8ie2
- Outcome
- growthe2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance”
Claim · model-written summary
Over-expression of SDG8i reduced senescence in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- constitutively over-expressing SDG8ie2
- Gene
- SDG8ie2
- Outcome
- senescencee2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance”
Claim · model-written summary
Over-expression of SDG8i increased cold tolerance of Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- constitutively over-expressing SDG8ie2
- Gene
- SDG8ie2
- Outcome
- cold tolerancee2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance”
Claim · model-written summary
Over-expression of SDG8i substantially improved protoplasmic drought tolerance of Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- constitutively over-expressing SDG8ie2
- Gene
- SDG8ie2
- Outcome
- protoplasmic drought tolerancee2
- Direction
- improvemente2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance”
Claim · model-written summary
Transgenic Arabidopsis over-expressing SDG8i showed large improvements in survival under cold, salt and drought stress.
- Taxon
- ArabidopsisArabidopsise5
- Treatment
- cold-, salt- and drought-stresse5
- Outcome
- survivale5
- Direction
- large improvementse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The large improvements observed in survival of transgenic Arabidopsis plants under cold-, salt- and drought-stress, as well as the substantial increases in growth rate and seed yield under non-stress conditions”
Claim · model-written summary
Over-expression of SDG8i increased growth rate and seed yield under non-stress conditions in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- constitutively over-expressing SDG8ie2
- Outcome
- growth rate and seed yielde5
- Direction
- increasese5
- Qualifier
- under non-stress conditionse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance”
e5“the substantial increases in growth rate and seed yield under non-stress conditions”
Claim · model-written summary
Plants over-expressing the UGT showed elevated auxin levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpressing the UGTe3
- Outcome
- auxin levelse3
- Direction
- elevatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis thaliana”
e3“Plants overexpressing the UGT show evidence of elevated auxin levels, with the enzyme acting downstream of ABA to reduce drought-induced senescence”
Claim · model-written summary
The UGT acts downstream of ABA to reduce drought-induced senescence in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Compound
- ABAe3
- Outcome
- drought-induced senescencee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis thaliana”
e3“Plants overexpressing the UGT show evidence of elevated auxin levels, with the enzyme acting downstream of ABA to reduce drought-induced senescence”
Claim · model-written summary
The recombinant SDG8i protein can glycosylate the strigolactone analogue GR24 in vitro.
- Taxon
- Sporobolus stapfianusSporobolus stapfianuse7
- Gene
- SDG8ie4
- Compound
- GR24e4
- Outcome
- can glycosylatee4
Verbatim
e7“the resurrection grass, Sporobolus stapfianus”
e4“Analysis of the in vitro activity of the UGT recombinant protein product demonstrates that SDG8i can glycosylate the synthetic strigolactone analogue GR24”