Abstract
The ankyrin repeat-containing protein gene OsPIANK1 (AK068021) in rice (Oryza sativa L.) was previously shown to be upregulated following infection with the rice leaf blight pathogen Xanthomonas oryzae pv oryzae (Xoo). In this study, we further characterized the role of OsPIANK1 in basal defense against Magnaporthe oryzae (M.oryzae) by 5' deletion analysis of its promoter and overexpression of the gene. The promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA). A TCA-element present in the region between -563 bp and -249 bp may be responsible for the OsPIANK1 response to both M.oryzae infection and exogenous SA application. The JERE box, CGTCA-box, and two MYB binding sites locating in the region between -1985 bp and -907 bp may be responsible for the response of OsPIANK1 to exogenous MeJA. OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA. Overexpression of OsPIANK1 enhanced resistance of rice to M.oryzae, although it did not confer complete resistance. The enhanced resistance to M.oryzae was accompanied by enhanced transcriptional expression of SA- and JA-dependent genes such as NH1, WKRY13, PAL, AOS2, PR1b, and PR5. This evidence suggests that OsPIANK1 acted as a positive regulator in rice basal defense mediated by SA- and JA-signaling pathways.
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Claims 12
Claim · model-written summary
The 1,985 bp OsPIANK1 promoter responded to inoculation with M.oryzae.
- Taxon
- rice
Oryza sativae0 - Treatment
- inoculation with M.oryzaee1
- Interactor
- M.oryzae
Pyricularia oryzaee1pathogen - Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 responsee1
- Direction
- inducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA)”
Claim · model-written summary
The 1,985 bp OsPIANK1 promoter responded to exogenous application of methyl jasmonate (MeJA).
- Taxon
- riceOryza sativae0
- Treatment
- exogenous application of methyl jasmonate (MeJA)e1
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 responsee1
- Direction
- inducee1
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA)”
Claim · model-written summary
The 1,985 bp OsPIANK1 promoter responded to salicylic acid (SA).
- Taxon
- riceOryza sativae0
- Treatment
- salicylic acid (SA)e1
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 responsee1
- Direction
- inducee1
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA)”
Claim · model-written summary
The 1,985 bp OsPIANK1 promoter did not respond to ABA.
- Taxon
- riceOryza sativae0
- Treatment
- exogenous application of abscisic acid (ABA)e1
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 responsee1
- Direction
- note1
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA)”
Claim · model-written summary
A TCA-element may mediate OsPIANK1 responses to M.oryzae infection and SA.
- Taxon
- riceOryza sativae0
- Interactor
- M.oryzaePyricularia oryzaee1pathogen
- Gene
- TCA-elemente1
- Outcome
- OsPIANK1 responsee1
- Qualifier
- may be responsiblee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“A TCA-element present in the region between -563 bp and -249 bp may be responsible for the OsPIANK1 response to both M.oryzae infection and exogenous SA application”
Claim · model-written summary
JERE, CGTCA and MYB sites may mediate the OsPIANK1 response to MeJA.
- Taxon
- riceOryza sativae0
- Gene
- JERE box, CGTCA-box, and two MYB binding sitese1
- Outcome
- response of OsPIANK1e1
- Qualifier
- may be responsiblee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“The JERE box, CGTCA-box, and two MYB binding sites locating in the region between -1985 bp and -907 bp may be responsible for the response of OsPIANK1 to exogenous MeJA”
Claim · model-written summary
OsPIANK1 expression rose after inoculation with M.oryzae.
- Taxon
- riceOryza sativae0
- Treatment
- inoculation with M.oryzaee1
- Interactor
- M.oryzaePyricularia oryzaee1pathogen
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 expressione1
- Direction
- upregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA”
Claim · model-written summary
OsPIANK1 expression rose after exogenous SA.
- Taxon
- riceOryza sativae0
- Treatment
- exogenous SAe1
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 expressione1
- Direction
- upregulatede1
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA”
Claim · model-written summary
OsPIANK1 expression rose after MeJA.
- Taxon
- riceOryza sativae0
- Treatment
- MeJAe1
- Gene
- OsPIANK1e1
- Outcome
- OsPIANK1 expressione1
- Direction
- upregulatede1
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA”
Claim · model-written summary
OsPIANK1 overexpression enhanced rice resistance to M.oryzae without complete resistance.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsPIANK1e1
- Interactor
- M.oryzaePyricularia oryzaee1pathogen
- Gene
- OsPIANK1e1
- Outcome
- resistance of rice to M.oryzaee1
- Direction
- enhancede1
- Qualifier
- did not confer complete resistancee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“Overexpression of OsPIANK1 enhanced resistance of rice to M.oryzae, although it did not confer complete resistance”
Claim · model-written summary
Enhanced resistance coincided with increased transcription of NH1, WKRY13, PAL, AOS2, PR1b and PR5.
- Taxon
- riceOryza sativae0
- Interactor
- M.oryzaePyricularia oryzaee1pathogen
- Gene
- NH1, WKRY13, PAL, AOS2, PR1b, and PR5e1
- Outcome
- transcriptional expressione1
- Direction
- enhancede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“enhanced resistance to M.oryzae was accompanied by enhanced transcriptional expression of SA- and JA-dependent genes such as NH1, WKRY13, PAL, AOS2, PR1b, and PR5”
Claim · model-written summary
The authors infer that OsPIANK1 positively regulates basal defense via SA and JA signaling.
- Taxon
- riceOryza sativae0
- Gene
- OsPIANK1e1
- Outcome
- rice basal defensee1
- Qualifier
- SA- and JA-signaling pathwayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa L.)”
e1“OsPIANK1 acted as a positive regulator in rice basal defense mediated by SA- and JA-signaling pathways”
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