Abstract
Response to the gaseous plant hormone ethylene in Arabidopsis requires the EIN3/EIL family of nuclear proteins. The biochemical function(s) of EIN3/EIL proteins, however, has remained unknown. In this study, we show that EIN3 and EILs comprise a family of novel sequence-specific DNA-binding proteins that regulate gene expression by binding directly to a primary ethylene response element (PERE) related to the tomato E4-element. Moreover, we identified an immediate target of EIN3, ETHYLENE-RESPONSE-FACTOR1 (ERF1), which contains this element in its promoter. EIN3 is necessary and sufficient for ERF1 expression, and, like EIN3-overexpression in transgenic plants, constitutive expression of ERF1 results in the activation of a variety of ethylene response genes and phenotypes. Evidence is also provided that ERF1 acts downstream of EIN3 and all other components of the ethylene signaling pathway. The results demonstrate that the nuclear proteins EIN3 and ERF1 act sequentially in a cascade of transcriptional regulation initiated by ethylene gas.
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Claims 6
Claim · model-written summary
EIN3 and EILs are sequence-specific DNA-binding proteins that directly bind PERE to regulate expression.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- EIN3 and EILse0
- Outcome
- gene expressione0
- Qualifier
- binding directly to a primary ethylene response element (PERE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EIN3 and EILs comprise a family of novel sequence-specific DNA-binding proteins that regulate gene expression by binding directly to a primary ethylene response element (PERE)”
e1“ethylene in Arabidopsis”
Claim · model-written summary
ERF1 was identified as an immediate EIN3 target containing PERE in its promoter.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- ETHYLENE-RESPONSE-FACTOR1 (ERF1)e0
- Outcome
- immediate target of EIN3e0
- Qualifier
- contains this element in its promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified an immediate target of EIN3, ETHYLENE-RESPONSE-FACTOR1 (ERF1), which contains this element in its promoter”
e1“ethylene in Arabidopsis”
Claim · model-written summary
EIN3 was necessary and sufficient for ERF1 expression.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- EIN3e0
- Outcome
- ERF1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EIN3 is necessary and sufficient for ERF1 expression”
e1“ethylene in Arabidopsis”
Claim · model-written summary
Constitutive ERF1 expression activated ethylene response genes and phenotypes.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- constitutive expression of ERF1e0
- Gene
- ERF1e0
- Outcome
- ethylene response genes and phenotypese0
- Direction
- activatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of ERF1 results in the activation of a variety of ethylene response genes and phenotypes”
e1“ethylene in Arabidopsis”
Claim · model-written summary
EIN3 overexpression activated ethylene response genes and phenotypes.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- EIN3-overexpressione0
- Gene
- EIN3e0
- Outcome
- ethylene response genes and phenotypese0
- Direction
- activatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“like EIN3-overexpression in transgenic plants, constitutive expression of ERF1 results in the activation of a variety of ethylene response genes and phenotypes”
e1“ethylene in Arabidopsis”
Claim · model-written summary
ERF1 acts downstream of EIN3 and other components in an ethylene-initiated transcriptional cascade.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- ERF1e0
- Outcome
- transcriptional regulatione1
- Qualifier
- downstream of EIN3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ERF1 acts downstream of EIN3 and all other components of the ethylene signaling pathway”
e1“EIN3 and ERF1 act sequentially in a cascade of transcriptional regulation initiated by ethylene gas”
e2“ethylene in Arabidopsis”
The same questions in other papers
overexpression · molecular: expression · ArabidopsisPositive and negative results
40 papers in the same direction as this paper · 14 in the opposite direction
- positive
- negative
- negative
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