Abstract
Explants from inflorescences of date palms from different female cultivars and one selected male clone were cultured on five growth regulator combinations. Shoot primordia formed mostly on Greshoff and Doy media containing: 0.5 mg L -1 of naphthalene acetic acid (NAA), 2 mg L -1 of 6-benzylaminopurine (BA) and 1 mg L -1 of 2-isopentylade- nine (2iP). Shoot multiplication occurred on the same medium, but plant growth and rooting were only obtained by a ten- fold reduction in cytokinin concentration or by an increase in NAA to 2 mg L -1 in combination with 1 mg L -1 2iP or 1 mg L -1 BA. Histological analysis of cultured explants revealed that buds originated from petal primordia. Percentages of reactive inflorescence explants in various media differed among cultivars examined. The technique offers great promise for vegetative propagation of date palms since several hundred plants could be obtained from a single inflores- cence, and each individual tree can produce several inflorescences annually. ( © Inra/Elsevier, Paris.) micropropagation / Phoenix dactylifera L. / organogenesis / female and male inflorescences Rsum -Multiplication vgtative du palmier dattier partir de segments d'inflorescences cultivs in vitro : effet de diffrentes combinaisons hormonales et capacits organogntiques de divers cultivars. Des fragments d'inflo- rescences appartenant diffrents cultivars femelles de palmier dattier et un clone mle slectionn ont t mis en cul- ture in vitro en prsence de cinq combinaisons hormonales diffrentes. La formation des bourgeons et leur multiplication taient meilleures sur le milieu Greshoff et Doy en presence de 0,5 mg L -1 d'acide naphtylactique (ANA), 2 mg L -1 de 6-benzylaminopurine (BAP) et 1 mg L -1 de 2-isopentyladnine (IPA). Cependant la rgnration de plantules entires n'a t obtenue qu'aprs rduction des teneurs en cytokinines ou augmentation des teneurs en ANA 2 mg L -1 en com- Communicated by Georges Pelletier (Versailles, France
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Claims 7
Claim · model-written summary
Shoot primordia formed mostly on Greshoff and Doy medium with NAA, BA and 2iP.
- Taxon
- date palms
Phoenix dactyliferat0 - Organ / stage
- Shoot primordiae0
- Treatment
- Greshoff and Doy media containing: 0.5 mg L -1 of naphthalene acetic acid (NAA), 2 mg L -1 of 6-benzylaminopurine (BA) and 1 mg L -1 of 2-isopentylade- nine (2iP)e0
- Outcome
- Shoot primordia formed mostlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot primordia formed mostly on Greshoff and Doy media containing: 0.5 mg L -1 of naphthalene acetic acid (NAA), 2 mg L -1 of 6-benzylaminopurine (BA) and 1 mg L -1 of 2-isopentylade- nine (2iP).”
t0“date palms”
Claim · model-written summary
Shoots multiplied on the same medium.
- Taxon
- date palmsPhoenix dactyliferat0
- Outcome
- Shoot multiplication occurrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot multiplication occurred on the same medium”
t0“date palms”
Claim · model-written summary
A tenfold cytokinin reduction enabled plant growth and rooting.
- Taxon
- date palmsPhoenix dactyliferat0
- Treatment
- ten- fold reduction in cytokinin concentratione0
- Outcome
- plant growth and rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant growth and rooting were only obtained by a ten- fold reduction in cytokinin concentration”
t0“date palms”
Claim · model-written summary
Increasing NAA to 2 mg L -1 with 2iP or BA enabled plant growth and rooting.
- Taxon
- date palmsPhoenix dactyliferat0
- Treatment
- increase in NAA to 2 mg L -1 in combination with 1 mg L -1 2iP or 1 mg L -1 BAe0
- Outcome
- plant growth and rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant growth and rooting were only obtained by a ten- fold reduction in cytokinin concentration or by an increase in NAA to 2 mg L -1 in combination with 1 mg L -1 2iP or 1 mg L -1 BA.”
t0“date palms”
Claim · model-written summary
Buds originated from petal primordia.
- Taxon
- date palmsPhoenix dactyliferat0
- Organ / stage
- petal primordiae0
- Outcome
- buds originatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“buds originated from petal primordia.”
t0“date palms”
Claim · model-written summary
The percentage of reactive inflorescence explants varied among cultivars and media.
- Taxon
- date palmsPhoenix dactyliferat0
- Material
- cultivars examinede0
- Treatment
- various mediae0
- Outcome
- Percentages of reactive inflorescence explantse0
- Direction
- differede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Percentages of reactive inflorescence explants in various media differed among cultivars examined.”
t0“date palms”
Claim · model-written summary
The technique could produce several hundred plants from one inflorescence.
- Taxon
- date palmsPhoenix dactyliferat0
- Outcome
- plants could be obtainede0
- Value
- several hundred plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several hundred plants could be obtained from a single inflores- cence”
t0“date palms”
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