Abstract
The accumulation of soluble carbohydrates resulting from growth under elevated CO2 may potentially signal the repression of gene activity for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS). To test this hypothesis we grew rice (Oryza sativa L.) under ambient (350 &mgr;L L-1) and high (700 &mgr;L L-1) CO2 in outdoor, sunlit, environment-controlled chambers and performed a cross-switching of growth CO2 concentration at the late-vegetative phase. Within 24 h, plants switched to high CO2 showed a 15% and 23% decrease in rbcS mRNA, whereas plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively. Ribulose-1,5-bisphosphate carboxylase/oxygenase total activity and protein content 8 d after the switch increased up to 27% and 20%, respectively, in plants switched to ambient CO2, but changed very little in plants switched to high CO2. Plants maintained at high CO2 showed greater carbohydrate pool sizes and lower rbcS transcript levels than plants kept at ambient CO2. However, after switching growth CO2 concentration, there was not a simple correlation between carbohydrate and rbcS transcript levels. We conclude that although carbohydrates may be important in the regulation of rbcS expression, changes in total pool size alone could not predict the rapid changes in expression that we observed.
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Claims 11
Claim · model-written summary
Switching to high CO2 decreased rbcS mRNA 15% in expanding rice leaves within 24 h.
- Taxon
- rice
Oryza sativae1 - Organ / stage
- expandinge0
- Treatment
- switched to high CO2e0
- Outcome
- rbcS mRNAe0
- Direction
- decreasee0
- Value
- 15%e0
- Comparator
- ambient CO2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Within 24 h, plants switched to high CO2 showed a 15% and 23% decrease in rbcS mRNA, whereas plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively.”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Switching to high CO2 decreased rbcS mRNA 23% in mature rice leaves within 24 h.
- Taxon
- riceOryza sativae1
- Organ / stage
- mature leavese0
- Treatment
- switched to high CO2e0
- Outcome
- rbcS mRNAe0
- Direction
- decreasee0
- Value
- 23%e0
- Comparator
- ambient CO2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Within 24 h, plants switched to high CO2 showed a 15% and 23% decrease in rbcS mRNA, whereas plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively.”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Switching to ambient CO2 increased rbcS mRNA 27% in expanding leaves.
- Taxon
- riceOryza sativae1
- Organ / stage
- expandinge0
- Treatment
- switched to ambient CO2e0
- Outcome
- rbcS mRNAe2
- Direction
- increasede0
- Value
- 27%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively.”
e1“rice (Oryza sativa L.)”
e2“rbcS mRNA”
Claim · model-written summary
Switching to ambient CO2 increased rbcS mRNA 11% in mature leaves.
- Taxon
- riceOryza sativae1
- Organ / stage
- mature leavese0
- Treatment
- switched to ambient CO2e0
- Outcome
- rbcS mRNAe2
- Direction
- increasede0
- Value
- 11%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively.”
e1“rice (Oryza sativa L.)”
e2“rbcS mRNA”
Claim · model-written summary
Rubisco total activity increased up to 27% eight days after switching to ambient CO2.
- Taxon
- riceOryza sativae1
- Treatment
- switched to ambient CO2e0
- Outcome
- Ribulose-1,5-bisphosphate carboxylase/oxygenase total activitye0
- Direction
- increasede0
- Value
- 27%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ribulose-1,5-bisphosphate carboxylase/oxygenase total activity and protein content 8 d after the switch increased up to 27% and 20%, respectively, in plants switched to ambient CO2”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Rubisco protein content increased up to 20% eight days after switching to ambient CO2.
- Taxon
- riceOryza sativae1
- Treatment
- switched to ambient CO2e0
- Outcome
- protein contente0
- Direction
- increasede0
- Value
- 20%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ribulose-1,5-bisphosphate carboxylase/oxygenase total activity and protein content 8 d after the switch increased up to 27% and 20%, respectively, in plants switched to ambient CO2”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Rubisco activity and protein content changed very little after switching to high CO2.
- Taxon
- riceOryza sativae2
- Treatment
- switched to high CO2e0
- Outcome
- total activity and protein contente1
- Direction
- changed very littlee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but changed very little in plants switched to high CO2.”
e1“Ribulose-1,5-bisphosphate carboxylase/oxygenase total activity and protein content”
e2“rice (Oryza sativa L.)”
Claim · model-written summary
Plants maintained at high CO2 had larger carbohydrate pools than ambient CO2 plants.
- Taxon
- riceOryza sativae1
- Treatment
- high CO2e0
- Outcome
- carbohydrate pool sizese0
- Direction
- greatere0
- Comparator
- ambient CO2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants maintained at high CO2 showed greater carbohydrate pool sizes and lower rbcS transcript levels than plants kept at ambient CO2.”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Plants maintained at high CO2 had lower rbcS transcript levels than ambient CO2 plants.
- Taxon
- riceOryza sativae1
- Treatment
- high CO2e0
- Outcome
- rbcS transcript levelse0
- Direction
- lowere0
- Comparator
- ambient CO2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants maintained at high CO2 showed greater carbohydrate pool sizes and lower rbcS transcript levels than plants kept at ambient CO2.”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
After switching CO2, carbohydrate and rbcS transcript levels lacked a simple correlation.
- Taxon
- riceOryza sativae1
- Compound
- carbohydratee0
- Outcome
- rbcS transcript levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after switching growth CO2 concentration, there was not a simple correlation between carbohydrate and rbcS transcript levels.”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
The authors conclude that total carbohydrate pool size alone could not predict rapid rbcS expression changes.
- Taxon
- riceOryza sativae1
- Compound
- carbohydratese2
- Outcome
- rapid changes in expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“changes in total pool size alone could not predict the rapid changes in expression that we observed.”
e1“rice (Oryza sativa L.)”
e2“carbohydrates may be important in the regulation of rbcS expression”
The same questions in other papers
co2 · molecular: expression · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- no effect
- no effect
4 papers more in this group
See all 8 papers →co2 · quality: protein · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 14 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.