Abstract
ABSTRACT Cobalt (Co) contamination in soils potentially affects human health through the food chain. Although rice ( Oryza sativa ) as a staple food is a major dietary source of human Co intake, it is poorly understood how Co is taken up by the roots and accumulated in rice grain. In this study, we physiologically characterized Co accumulation and identified the transporter for Co 2+ uptake in rice. A dose‐dependent experiment showed that Co mainly accumulated in rice roots. Further analysis with LA‐ICP‐MS showed Co deposited in most tissue of the roots, including exodermis, endodermis and stele region. Co accumulation analysis using mutants defective in divalent cation uptake showed that Co 2+ uptake in rice is mediated by the Mn 2+ /Cd 2+ /Pb 2+ transporter OsNramp5, rather than OsIRT1 for Fe 2+ and OsZIP9 for Zn 2+ . Knockout of OsNramp5 enhanced tolerance to Co toxicity. Heterologous expression of OsNramp5 showed transport activity for Co 2+ in Saccharomyces cerevisiae . Co 2+ uptake was inhibited by either Mn 2+ or Cd 2+ supply. At the reproductive stage, the Co concentration in the straw and grains of the OsNramp5 knockout lines was decreased by 41%–48% and 28%–36%, respectively, compared with that of the wild‐type rice. The expression level of OsNramp5 in the roots was not affected by Co 2+ . Taken together, our results indicate that OsNramp5 is a major transporter for Co 2+ uptake in rice, which ultimately mediates Co accumulation in the grains.
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Claims 10
Claim · model-written summary
Cobalt accumulated mainly in rice roots in a dose-dependent experiment.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- rice rootse0
- Outcome
- Co mainly accumulated in rice rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“A dose‐dependent experiment showed that Co mainly accumulated in rice roots”
Claim · model-written summary
Cobalt was deposited in root exodermis, endodermis and stele.
- Taxon
- RiceOryza sativat0
- Organ / stage
- exodermis, endodermis and stele regione0
- Outcome
- Co deposited in most tissue of the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co deposited in most tissue of the roots, including exodermis, endodermis and stele region”
Claim · model-written summary
Rice cobalt uptake was mediated by OsNramp5 rather than OsIRT1 or OsZIP9.
- Taxon
- RiceOryza sativat0
- Gene
- OsNramp5e0
- Outcome
- Co 2+ uptake in ricee0
- Comparator
- OsIRT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co 2+ uptake in rice is mediated by the Mn 2+ /Cd 2+ /Pb 2+ transporter OsNramp5, rather than OsIRT1 for Fe 2+ and OsZIP9 for Zn 2+”
Claim · model-written summary
Knocking out OsNramp5 increased rice tolerance to cobalt toxicity.
- Taxon
- RiceOryza sativat0
- Treatment
- Knockout of OsNramp5e0
- Gene
- OsNramp5e0
- Outcome
- tolerance to Co toxicitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Knockout of OsNramp5 enhanced tolerance to Co toxicity”
Claim · model-written summary
OsNramp5 transported cobalt in heterologous yeast expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsNramp5e0
- Outcome
- transport activity for Co 2+e0
- Qualifier
- Saccharomyces cerevisiaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Heterologous expression of OsNramp5 showed transport activity for Co 2+ in Saccharomyces cerevisiae”
Claim · model-written summary
Manganese supply inhibited cobalt uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- Mn 2+e0
- Outcome
- Co 2+ uptakee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co 2+ uptake was inhibited by either Mn 2+ or Cd 2+ supply”
Claim · model-written summary
Cadmium supply inhibited cobalt uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- Cd 2+e0
- Outcome
- Co 2+ uptakee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co 2+ uptake was inhibited by either Mn 2+ or Cd 2+ supply”
Claim · model-written summary
OsNramp5 knockout reduced straw cobalt by 41%–48% versus wild type.
- Taxon
- RiceOryza sativat0
- Material
- OsNramp5 knockout linese0
- Organ / stage
- strawe0
- Treatment
- OsNramp5 knockout linese0
- Outcome
- Co concentration in the strawe0
- Direction
- decreasede0
- Value
- 41%–48%e0
- Comparator
- wild‐type ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co concentration in the straw and grains of the OsNramp5 knockout lines was decreased by 41%–48% and 28%–36%, respectively, compared with that of the wild‐type rice”
Claim · model-written summary
OsNramp5 knockout reduced grain cobalt by 28%–36% versus wild type.
- Taxon
- RiceOryza sativat0
- Material
- OsNramp5 knockout linese0
- Organ / stage
- grainse0
- Treatment
- OsNramp5 knockout linese0
- Outcome
- Co concentration in the straw and grainse0
- Direction
- decreasede0
- Value
- 28%–36%e0
- Comparator
- wild‐type ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Co concentration in the straw and grains of the OsNramp5 knockout lines was decreased by 41%–48% and 28%–36%, respectively, compared with that of the wild‐type rice”
Claim · model-written summary
Root OsNramp5 expression was unaffected by cobalt.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse0
- Treatment
- Co 2+e0
- Gene
- OsNramp5e0
- Outcome
- expression level of OsNramp5 in the rootse0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“The expression level of OsNramp5 in the roots was not affected by Co 2+”
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See all 154 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.