Abstract
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.
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Claims 11
Claim · model-written summary
Loss of function in OsAPX2 in rice produced semi-dwarf seedlings, yellow-green leaves and leaf lesion mimic
- Taxon
- rice
Oryza sativae1 - Material
- Osapx2 knockout mutante2
- Treatment
- Loss of functione1
- Gene
- OsAPX2e1
- Outcome
- semi-dwarf seedlings, yellow-green leaves, leaf lesion mimice1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
e2“The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment”
Claim · model-written summary
Loss of function in OsAPX2 in rice caused seed sterility
- Taxon
- riceOryza sativae1
- Treatment
- Loss of functione1
- Gene
- OsAPX2e1
- Outcome
- seed sterilitye1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
OsAPX2 expression in rice is developmentally and spatially regulated
- Taxon
- riceOryza sativae2
- Gene
- OsAPX2e1
- Outcome
- OsAPX2 expressione1
- Qualifier
- developmental- and spatial-regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses”
e2“The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment”
Claim · model-written summary
OsAPX2 expression in rice is induced by drought, salt and cold stresses
- Taxon
- riceOryza sativae2
- Treatment
- drought, salt, and cold stressese1
- Gene
- OsAPX2e1
- Outcome
- OsAPX2 expressione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses”
e2“The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment”
Claim · model-written summary
Osapx2 mutants of rice had lower APX activity
- Taxon
- riceOryza sativae2
- Material
- Osapx2 mutantse1
- Treatment
- Loss of functione2
- Outcome
- APX activitye1
- Direction
- lowere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
Osapx2 mutants of rice were sensitive to abiotic stresses
- Taxon
- riceOryza sativae2
- Material
- Osapx2 mutantse1
- Treatment
- abiotic stressese1
- Outcome
- sensitive to abiotic stressese1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
Overexpression of OsAPX2 in rice increased APX activity
- Taxon
- riceOryza sativae2
- Treatment
- overexpression of OsAPX2e1
- Gene
- OsAPX2e1
- Outcome
- APX activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of OsAPX2 increased APX activity and enhanced stress tolerance”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
Overexpression of OsAPX2 in rice enhanced stress tolerance
- Taxon
- riceOryza sativae2
- Treatment
- overexpression of OsAPX2e1
- Gene
- OsAPX2e1
- Outcome
- stress tolerancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of OsAPX2 increased APX activity and enhanced stress tolerance”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
H2O2 and MDA levels were high in Osapx2 mutants of rice relative to wild-type plants after stress treatments
- Taxon
- riceOryza sativae2
- Material
- Osapx2 mutantse1
- Treatment
- stress treatmentse1
- Outcome
- H2O2 and MDA levelse1
- Direction
- highe1
- Comparator
- wild-type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
H2O2 and MDA levels were low in OsAPX2-OX transgenic rice lines relative to wild-type plants after stress treatments
- Taxon
- riceOryza sativae2
- Material
- OsAPX2-OX transgenic linese1
- Treatment
- stress treatmentse1
- Outcome
- H2O2 and MDA levelse1
- Direction
- lowe1
- Comparator
- wild-type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments”
e2“Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility”
Claim · model-written summary
OsAPX2 plays an important role in rice growth and development by protecting seedlings from abiotic stresses through scavenging reactive oxygen species
- Taxon
- riceOryza sativae1
- Gene
- OsAPX2e1
- Outcome
- protecting the seedlings from abiotic stressese1
- Qualifier
- scavenging reactive oxygen speciese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species”
The same questions in other papers
loss of function · disease · Oryza sativa (rice)Positive and negative results
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