Paper · 1969 · American Journal of Botany

A HISTOCHEMICAL STUDY OF ABSCISSION LAYER FORMATION IN THE BEAN

H. P. Rasmussen; M. J. Bukovac

14
claims
28
verbatim quotes
21
citations
2
research areas
—
Not open access

Abstract

In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr. Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole. Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading. The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls. This was followed by breakdown of other cell wall components, i.e., non‐cellulosic polysaccharides and cellulose. The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered; however, no lignin was present during abscission layer development. The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation. Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight.

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Claims 14

Observationobservedoriginal resultmeasured

Claim · model-written summary

Calcium increased in the abscission zone of debladed bean petioles during the 14 hr induction period.

Taxon
BEANt0
Organ / stage
abscission zonee0
Outcome
calciume0
Direction
increasede0
Value
14 hre0

Verbatim

e0

“In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dry weight increased in the abscission zone during the 14 hr induction period.

Taxon
BEANt0
Organ / stage
abscission zonee0
Outcome
dry weighte0
Direction
increasede0
Value
14 hre0

Verbatim

e0

“In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before layer appearance, calcium decreased in the abscission zone.

Taxon
BEANt0
Organ / stage
abscission zonee0
Outcome
calciume0
Direction
decreasede0

Verbatim

e0

“Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole”

✓ located verbatim in the source · abstract, characters 119–246 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before layer appearance, calcium increased in the petiole.

Taxon
BEANt0
Organ / stage
petiolee0
Outcome
calciume0
Direction
increasede0

Verbatim

e0

“Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole”

✓ located verbatim in the source · abstract, characters 119–246 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dry matter decreased in abscission zone and petiole 24 hr after deblading.

Taxon
BEANt0
Organ / stage
abscission zone and the petiolee0
Outcome
Dry mattere0
Direction
decreasee0
Value
24 hre0

Verbatim

e0

“Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading”

✓ located verbatim in the source · abstract, characters 248–342 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pectic material swelling was the first visual change in abscission zone cells.

Taxon
BEANt0
Organ / stage
abscission zonee0
Outcome
swelling of the pectic materials of the cell wallse0

Verbatim

e0

“The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls”

✓ located verbatim in the source · abstract, characters 344–460 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Non-cellulosic polysaccharides and cellulose subsequently broke down.

Taxon
BEANt0
Outcome
breakdown of other cell wall components, i.e., non‐cellulosic polysaccharides and cellulosee0

Verbatim

e0

“This was followed by breakdown of other cell wall components, i.e., non‐cellulosic polysaccharides and cellulose”

✓ located verbatim in the source · abstract, characters 462–574 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cellulose adjacent and distal to the layer was altered.

Taxon
BEANt0
Outcome
cellulose of the cell walls adjacent and distal to the abscission layere0
Qualifier
alterede0

Verbatim

e0

“The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered”

✓ located verbatim in the source · abstract, characters 576–675 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No lignin was present during layer development.

Taxon
BEANt0
Organ / stage
abscission layer developmente0
Outcome
no lignin was presente0

Verbatim

e0

“no lignin was present during abscission layer development”

✓ located verbatim in the source · abstract, characters 686–743 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Mechanismobservedoriginal resultmeasured

Claim · model-written summary

Cell wall component changes caused abscission layer cells to collapse before separation.

Taxon
BEANt0
Outcome
collapse of cells of the abscission layere0
Qualifier
alteration of pectic materials, coupled with breakdown of cell wall componentse0

Verbatim

e0

“The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation”

✓ located verbatim in the source · abstract, characters 745–911 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed abscission in bean petioles.

Taxon
BEANt0
Treatment
Auxine0
Outcome
abscissione0
Direction
delayede0

Not reportedComparator

Verbatim

e0

“Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight”

✓ located verbatim in the source · abstract, characters 913–1083 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed initial increase in calcium in bean petioles.

Taxon
BEANt0
Treatment
Auxine0
Outcome
initial increase in calciume0
Direction
delayede0

Not reportedComparator

Verbatim

e0

“Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight”

✓ located verbatim in the source · abstract, characters 913–1083 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed movement of calcium from the abscission zone to the petiole in bean petioles.

Taxon
BEANt0
Treatment
Auxine0
Outcome
movement of calcium from the abscission zone to the petiolee0
Direction
delayede0

Not reportedComparator

Verbatim

e0

“Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight”

✓ located verbatim in the source · abstract, characters 913–1083 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed decrease in dry weight in bean petioles.

Taxon
BEANt0
Treatment
Auxine0
Outcome
decrease in dry weighte0
Direction
delayede0

Not reportedComparator

Verbatim

e0

“Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight”

✓ located verbatim in the source · abstract, characters 913–1083 · show in text ↑
t0

“THE BEAN”

✓ located verbatim in the source · title, characters 55–63 · show in text ↑