Paper · 1978 · Journal of Experimental Botany

Inorganic Nitrogen Metabolism in Barley Roots Under Poorly Aerated Conditions

R. B. LEE

7
claims
15
verbatim quotes
57
citations
1
research areas
—
Not open access

Abstract

Nitrate reduction, nitrite reduction, and ammonium assimilation were measured over c. 24 h in excised sterile barley roots, in air or under low oxygen tensions. Partial anoxia had relatively little effect, but the pathway of nitrogen assimilation was severely inhibited during complete anoxia, when the uptake of nitrate ceased. Much of the nitrate which was present in the roots at the time of excision was apparently unavailable for assimilation. None of the reactions of the pathway served in place of oxygen as an electron acceptor under anaerobic conditions. The concentration of nitrate in the external solution during growth and during the experimental treatments had no direct effect on anaerobic ethanol formation, although an indirect effect was noted which was due to variations in the carbohydrate content of the tissue.

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Claims 7

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Partial anoxia had relatively little effect on barley root nitrogen assimilation.

Treatment
Partial anoxiae0
Outcome
effecte0
Direction
little effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Partial anoxia had relatively little effect”

✓ located verbatim in the source · abstract, characters 161–204 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Complete anoxia severely inhibited the pathway of nitrogen assimilation.

Treatment
complete anoxiae0
Outcome
pathway of nitrogen assimilatione0
Direction
severely inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the pathway of nitrogen assimilation was severely inhibited during complete anoxia”

✓ located verbatim in the source · abstract, characters 210–292 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Nitrate uptake ceased during complete anoxia.

Treatment
complete anoxiae0
Outcome
uptake of nitratee0
Direction
ceasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“during complete anoxia, when the uptake of nitrate ceased”

✓ located verbatim in the source · abstract, characters 270–327 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Much of the nitrate in roots at excision appeared unavailable for assimilation.

Organ / stage
rootse0
Outcome
unavailable for assimilatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Much of the nitrate which was present in the roots at the time of excision was apparently unavailable for assimilation”

✓ located verbatim in the source · abstract, characters 329–447 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No nitrogen assimilation reaction substituted for oxygen as an electron acceptor anaerobically.

Outcome
served in place of oxygen as an electron acceptore0
Qualifier
under anaerobic conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“None of the reactions of the pathway served in place of oxygen as an electron acceptor under anaerobic conditions”

✓ located verbatim in the source · abstract, characters 449–562 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

External nitrate concentration had no direct effect on anaerobic ethanol formation.

Treatment
concentration of nitrate in the external solutione0
Outcome
anaerobic ethanol formatione0
Direction
no direct effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The concentration of nitrate in the external solution during growth and during the experimental treatments had no direct effect on anaerobic ethanol formation”

✓ located verbatim in the source · abstract, characters 564–722 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

External nitrate concentration indirectly affected anaerobic ethanol formation through tissue carbohydrate content.

Compound
carbohydratee0
Outcome
anaerobic ethanol formatione1
Qualifier
variations in the carbohydrate content of the tissuee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“an indirect effect was noted which was due to variations in the carbohydrate content of the tissue”

✓ located verbatim in the source · abstract, characters 733–831 · show in text ↑
e1

“anaerobic ethanol formation”

✓ located verbatim in the source · abstract, characters 695–722 · show in text ↑
t0

“Barley Roots”

✓ located verbatim in the source · title, characters 33–45 · show in text ↑

nitrogen · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results

31 papers: 15 positive, 16 negative or no effect

15 papers in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

27 papers more in this group

See all 31 papers →

flooding · nutrient: content · Hordeum vulgare (barley)Results agree

9 papers: 0 positive, 9 negative or no effect

8 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.