Abstract
Abstract Control dormant seeds that imbibed water for 16 hr germinated 100% after 10 μ 1/1 C2H4 was applied for 24 hr. Dormant seeds that imbibed cycloheximide (100 μg/ml), 6-methylpurine (50 μ g/ml) and 6-azauracil (50 μ g/ml) for 16 hr did not germinate at either 24 or 48 hr after 10 μ 1/1 ethylene treatment. Both protein- and nucleic acid-synthesis ihhibitors prevented germination induced by ethylene in these dormant seeds. Imbibition of 20 μ M ABA by dormant seeds prevented germination, but this effect was reversed by ethylene. Tracer studies with 14C-amino acids indicate that ABA does not inhibit total protein synthesis, but it does inhibit emergence in the absence of ethylene. In the presence of ABA plus ethylene, emergence occurred, but no change in total protein synthesis was detected. At 8 weeks after harvest, both germination and incorporation of 2–14C-uracil into RNA were inhibited by ABA and stimulated by ethylene. By 17 weeks after harvest, only the inhibition of germination and its reversal by ethylene were notable. However, at 17 weeks after harvest, ethylene enhanced RNA synthesis when germination and protein synthesis were inhibited by cycloheximide. Development of isocitritase activity in the seeds was inhibited by ABA and the inhibition was reversed by ethylene, indicating that de novo synthesis of protein is inhibited by ABA and activated by ethylene in these seeds. The opposite effects of ABA and ethylene on germination, RNA synthesis and isocitritase activity suggest that germination is controlled at the level of RNA and/or protein synthesis in these seeds. The prevention of germination of dormant seeds in the presence of ethylene by protein- and RNA-synthesis ihhibitors supports this suggestion, but the data do not preclude an action of ABA or ethylene prior to detectable affects on RNA or protein synthesis.
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Claims 13
Claim · model-written summary
Ethylene induced 100% germination of water-imbibed dormant seeds.
- Taxon
- Peanut
Arachis hypogaeat0 - Organ / stage
- dormant seedse0
- Treatment
- 10 μ 1/1 C2H4e0
- Outcome
- germinatede0
- Direction
- germinatede0
- Value
- 100%e0
- Comparator
- Control dormant seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Control dormant seeds that imbibed water for 16 hr germinated 100% after 10 μ 1/1 C2H4 was applied for 24 hr.”
Claim · model-written summary
Cycloheximide, 6-methylpurine and 6-azauracil prevented ethylene-induced germination.
- Taxon
- PeanutArachis hypogaeat0
- Organ / stage
- Dormant seedse0
- Treatment
- cycloheximide (100 μg/ml), 6-methylpurine (50 μ g/ml) and 6-azauracil (50 μ g/ml)e0
- Outcome
- did not germinatee0
- Comparator
- 10 μ 1/1 ethylene treatmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Dormant seeds that imbibed cycloheximide (100 μg/ml), 6-methylpurine (50 μ g/ml) and 6-azauracil (50 μ g/ml) for 16 hr did not germinate at either 24 or 48 hr after 10 μ 1/1 ethylene treatment.”
Claim · model-written summary
ABA prevented dormant seed germination.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- 20 μ M ABAe0
- Outcome
- germinatione0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Imbibition of 20 μ M ABA by dormant seeds prevented germination, but this effect was reversed by ethylene.”
Claim · model-written summary
Ethylene reversed ABA inhibition of germination.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ethylenee0
- Outcome
- germinatione0
- Direction
- reversede0
- Comparator
- 20 μ M ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Imbibition of 20 μ M ABA by dormant seeds prevented germination, but this effect was reversed by ethylene.”
Claim · model-written summary
ABA did not inhibit total protein synthesis.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ABAe0
- Outcome
- total protein synthesise0
- Direction
- does not inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“ABA does not inhibit total protein synthesis, but it does inhibit emergence in the absence of ethylene.”
Claim · model-written summary
ABA inhibited emergence without ethylene.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ABAe0
- Outcome
- emergencee0
- Direction
- inhibite0
- Comparator
- absence of ethylenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“ABA does not inhibit total protein synthesis, but it does inhibit emergence in the absence of ethylene.”
Claim · model-written summary
With ABA and ethylene, emergence occurred without a detectable change in total protein synthesis.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ABA plus ethylenee0
- Outcome
- emergence occurrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“In the presence of ABA plus ethylene, emergence occurred, but no change in total protein synthesis was detected.”
Claim · model-written summary
At eight weeks, ABA inhibited germination and RNA incorporation.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ABAe0
- Outcome
- germination and incorporation of 2–14C-uracil into RNAe0
- Direction
- inhibitede0
- Qualifier
- At 8 weeks after harveste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“At 8 weeks after harvest, both germination and incorporation of 2–14C-uracil into RNA were inhibited by ABA and stimulated by ethylene.”
Claim · model-written summary
At eight weeks, ethylene stimulated germination and RNA incorporation.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ethylenee0
- Outcome
- germination and incorporation of 2–14C-uracil into RNAe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“At 8 weeks after harvest, both germination and incorporation of 2–14C-uracil into RNA were inhibited by ABA and stimulated by ethylene.”
Claim · model-written summary
At 17 weeks, ABA inhibited germination and ethylene reversed it.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- inhibition of germination and its reversal by ethylenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“By 17 weeks after harvest, only the inhibition of germination and its reversal by ethylene were notable.”
Claim · model-written summary
Ethylene enhanced RNA synthesis at 17 weeks despite cycloheximide inhibition of germination and protein synthesis.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ethylenee0
- Outcome
- RNA synthesise0
- Direction
- enhancede0
- Qualifier
- when germination and protein synthesis were inhibited by cycloheximidee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“at 17 weeks after harvest, ethylene enhanced RNA synthesis when germination and protein synthesis were inhibited by cycloheximide.”
Claim · model-written summary
ABA inhibited seed isocitritase activity development.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ABAe0
- Gene
- isocitritasee0
- Outcome
- Development of isocitritase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Development of isocitritase activity in the seeds was inhibited by ABA and the inhibition was reversed by ethylene”
Claim · model-written summary
Ethylene reversed ABA inhibition of seed isocitritase activity.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- ethylenee0
- Outcome
- isocitritase activitye0
- Direction
- reversede0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“NC-13 Virginia-type Peanut Seeds”
e0“Development of isocitritase activity in the seeds was inhibited by ABA and the inhibition was reversed by ethylene”
The same questions in other papers
(+)-abscisic acid · growth · Arachis hypogaea (peanut)Results differ
3 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →(+)-abscisic acid · quality: protein · Arachis hypogaea (peanut)Results agree
1 paper in the same direction as this paper
- no effect
cycloheximide · growth · Arachis hypogaea (peanut)Results agree
2 papers in the same direction as this paper
- negative
- negative
ethene · growth · Arachis hypogaea (peanut)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.