Abstract
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.
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Claims 16
Claim · model-written summary
Hv-SGT1 was isolated and characterized from Haynaldia villosa.
Verbatim
e0“we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV)”
Claim · model-written summary
Hv-SGT1 was located in the cytoplasm and nucleus.
- Taxon
- Haynaldia villosa
Dasypyrum villosumt0 - Gene
- Hv-SGT1t0
- Outcome
- presence in the cytoplasm and nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicated its presence in the cytoplasm and nucleus”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Bgt inoculation increased Hv-SGT1 transcripts.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt)e0
- Interactor
- Blumeriagraminis DC. f. Sp. triticie0pathogen
- Gene
- Hv-SGT1e0
- Outcome
- Levels of Hv-SGT1 transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg)”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Fg inoculation increased Hv-SGT1 transcripts.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- Fusariumgraminearum (Fg)e0
- Interactor
- Fusariumgraminearume0pathogen
- Gene
- Hv-SGT1e0
- Outcome
- Levels of Hv-SGT1 transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg)”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
H2O2 treatment substantially increased Hv-SGT1 levels.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- H2O2e0
- Gene
- Hv-SGT1e0
- Outcome
- Levels of Hv-SGT1e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA)”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Methyl jasmonate substantially increased Hv-SGT1 levels.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- methyl jasmonate (MeJA)e0
- Gene
- Hv-SGT1e0
- Outcome
- Levels of Hv-SGT1e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA)”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Ethephon slightly induced Hv-SGT1 levels.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- ethephone0
- Gene
- Hv-SGT1t0
- Outcome
- Hv-SGT1t0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only slightly induced following exposure to ethephon or abscisic acid”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Abscisic acid slightly induced Hv-SGT1 levels.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- abscisic acide0
- Gene
- Hv-SGT1t0
- Outcome
- Hv-SGT1t0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only slightly induced following exposure to ethephon or abscisic acid”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Salicylic acid did not change Hv-SGT1 levels.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- salicylic acide0
- Gene
- Hv-SGT1t0
- Outcome
- Hv-SGT1t0
- Direction
- not changede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not changed following exposure to salicylic acid”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 silencing substantially reduced resistance to Bgt.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- silencing of Hv-SGT1e0
- Gene
- Hv-SGT1e0
- Outcome
- resistance to Bgte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of Hv-SGT1 substantially reduced resistance to Bgt”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 overexpression enhanced powdery mildew resistance in Yangmai 158.
- Taxon
- Common Wheat
Triticum aestivumt0 - Material
- Yangmai 158e0
- Treatment
- over-expression of Hv-SGT1e0
- Interactor
- powdery mildewe0pathogen
- Gene
- Hv-SGT1e0
- Outcome
- resistance to powdery mildewe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 overexpression correlated with increased reactive oxygen intermediates at pathogen penetration sites.
- Taxon
- Common WheatTriticum aestivumt0
- Gene
- Hv-SGT1t0
- Outcome
- levels of whole-cell reactive oxygen intermediatese0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 silencing lowered expression of H2O2 and JA signaling genes versus wild type.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Treatment
- Hv-SGT1 silenced plantse0
- Gene
- Hv-SGT1e0
- Outcome
- expression levels of genes related to the H2O2 and JA signaling pathwayse0
- Direction
- lowere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 overexpression raised expression of H2O2 and JA signaling genes versus wild type.
- Taxon
- Common WheatTriticum aestivumt0
- Treatment
- Hv-SGT1 over-expressing plantse0
- Gene
- Hv-SGT1e0
- Outcome
- expression levels of genes related to the H2O2 and JA signaling pathwayse0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Wheat overexpressing Hv-SGT1 had enhanced powdery mildew resistance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- over-expressed Hv-SGT1e0
- Interactor
- powdery mildewe0pathogen
- Gene
- Hv-SGT1e0
- Outcome
- resistance to both powdery mildew and FHBe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
Claim · model-written summary
Hv-SGT1 overexpressing wheat was suggested as a breeding resource for multiple disease resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- Hv-SGT1t0
- Outcome
- transgenic genetic resource in wheat breeding for multiple disease resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance”
t0“The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)”
The same questions in other papers
inoculation · molecular: expression · Dasypyrum villosumPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
loss of function · disease · Dasypyrum villosumResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.