Abstract
Powdery mildew is one of the most devastating wheat fungal diseases. A diploid wheat relative, Haynaldia villosa L., is highly resistant to powdery mildew, and its genetic resource of resistances, such as the Pm21 locus, is now widely used in wheat breeding. Here we report the cloning of a resistance gene from H. villosa, designated CMPG1-V, that encodes a U-box E3 ubiquitin ligase. Expression of the CMPG1-V gene was induced in the leaf and stem of H. villosa upon inoculation with Blumeria graminis f. sp. tritici (Bgt) fungus, and the presence of Pm21 is essential for its rapid induction of expression. CMPG1-V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo. CMPG1-V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesicles. Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes, H2 O2 accumulation, and cell-wall protein cross-linking at the Bgt infection sites, and the expression of CMPG1-V in H. villosa was increased when treated with salicylic acid, abscisic acid and H2 O2 . These results indicate the involvement of E3 ligase in defense responses to Bgt fungus in wheat, particularly in broad-spectrum disease resistance, and suggest association of reactive oxidative species and the phytohormone pathway with CMPG1-V-mediated powdery mildew resistance.
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Claims 12
Claim · model-written summary
A CMPG1-V resistance gene encoding a U-box E3 ubiquitin ligase was cloned from H. villosa.
- Taxon
- H. villosa
Dasypyrum villosume0 - Gene
- CMPG1-Ve0
- Outcome
- resistance genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cloning of a resistance gene from H. villosa, designated CMPG1-V, that encodes a U-box E3 ubiquitin ligase”
Claim · model-written summary
Bgt inoculation induced CMPG1-V expression in H. villosa leaves and stems.
- Taxon
- H. villosaDasypyrum villosume0
- Organ / stage
- leaf and steme0
- Treatment
- inoculation with Blumeria graminis f. sp. tritici (Bgt) funguse0
- Interactor
- Blumeria graminis f. sp. tritici (Bgt)
Blumeria graminis f. sp. triticie0pathogen - Gene
- CMPG1-Ve0
- Outcome
- Expression of the CMPG1-V genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the CMPG1-V gene was induced in the leaf and stem of H. villosa upon inoculation with Blumeria graminis f. sp. tritici (Bgt) fungus”
Claim · model-written summary
The Pm21 locus is essential for rapid induction of CMPG1-V expression.
- Taxon
- H. villosaDasypyrum villosume1
- Gene
- Pm21e0
- Outcome
- rapid induction of expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of Pm21 is essential for its rapid induction of expression”
e1“Expression of the CMPG1-V gene was induced in the leaf and stem of H. villosa”
Claim · model-written summary
CMPG1-V has conserved E3 ligase residues and E3 ligase activity in vitro and in vivo.
- Taxon
- Haynaldia villosa L.Dasypyrum villosumt0
- Gene
- CMPG1-Ve0
- Outcome
- E3 ligase activitye0
- Setting
- in vitro and in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMPG1-V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo”
t0“Haynaldia villosa L.”
Claim · model-written summary
CMPG1-V localized to nucleus, endoplasmic reticulum, plasma membrane and some vesicles.
- Taxon
- Haynaldia villosa L.Dasypyrum villosumt0
- Gene
- CMPG1-Ve0
- Outcome
- localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesiclese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMPG1-V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesicles”
t0“Haynaldia villosa L.”
Claim · model-written summary
CMPG1-V overexpression improved broad spectrum powdery mildew resistance in wheat at seedling and adult stages.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- seedling and adult stagese0
- Treatment
- over-expressing CMPG1-Ve0
- Gene
- CMPG1-Ve0
- Outcome
- powdery mildew resistancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages”
Claim · model-written summary
CMPG1-V overexpression was associated with increased expression of salicylic acid responsive genes.
- Taxon
- wheatTriticum aestivume0
- Gene
- CMPG1-Ve0
- Outcome
- expression of salicylic acid-responsive genese0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes”
Claim · model-written summary
CMPG1-V overexpression was associated with H2 O2 accumulation at Bgt infection sites.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Bgte0pathogen
- Gene
- CMPG1-Ve0
- Outcome
- H2 O2 accumulatione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes, H2 O2 accumulation, and cell-wall protein cross-linking at the Bgt infection sites”
Claim · model-written summary
CMPG1-V overexpression was associated with cell wall protein cross linking at Bgt infection sites.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Bgte0pathogen
- Gene
- CMPG1-Ve1
- Outcome
- cell-wall protein cross-linkinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell-wall protein cross-linking at the Bgt infection sites”
e1“Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance”
Claim · model-written summary
Salicylic acid treatment increased CMPG1-V expression in H. villosa.
- Taxon
- H. villosaDasypyrum villosume0
- Treatment
- salicylic acide0
- Gene
- CMPG1-Ve0
- Outcome
- expression of CMPG1-Ve0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of CMPG1-V in H. villosa was increased when treated with salicylic acid, abscisic acid and H2 O2”
Claim · model-written summary
Abscisic acid treatment increased CMPG1-V expression in H. villosa.
- Taxon
- H. villosaDasypyrum villosume0
- Treatment
- abscisic acide0
- Gene
- CMPG1-Ve0
- Outcome
- expression of CMPG1-Ve0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of CMPG1-V in H. villosa was increased when treated with salicylic acid, abscisic acid and H2 O2”
Claim · model-written summary
H2 O2 treatment increased CMPG1-V expression in H. villosa.
- Taxon
- H. villosaDasypyrum villosume0
- Treatment
- H2 O2e0
- Gene
- CMPG1-Ve0
- Outcome
- expression of CMPG1-Ve0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of CMPG1-V in H. villosa was increased when treated with salicylic acid, abscisic acid and H2 O2”
The same questions in other papers
inoculation · molecular: expression · Dasypyrum villosumPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.