Abstract
Hv-S/TPK gene, a resistance related gene to powdery mildew, was cloned by using genechip, and its expression was upregulated after the inoculation of Blumeria graminis to Haynaldia villosa. Using the specific primers of Hv-S/TPK to screen a genomic TAC (Transformation-competent artificial chromosome) library of translocation line 6VS/6AL, a positive TAC was screened. A 5-kb fragment containing Hv-S/TPK was subcloned and identified. This 5160-bp fragment (GenBank Accession No. EU153366) was determined by specific primer walking. The analysis of Hv-S/TPK genomic sequence showed three introns and four extrons between start code and stop code. In the promoter region of Hv-S/TPK, there were W-box and OCS-like elements which were the elements related to disease resistance. In this study, the positive TAC clone was used to as probe in situ hybridized to mitotic metaphase chromosomes of translocation line. The result of fluorescence in situ hybridization (FISH) indicated that the TAC clone containing Hv-S/TPK was from Haynaldia villosa chromosome.
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Claims 7
Claim · model-written summary
Hv-S/TPK expression rose after Blumeria graminis inoculation of Haynaldia villosa.
- Taxon
- Haynaldia villosa
Dasypyrum villosume0 - Treatment
- inoculation of Blumeria graminise0
- Interactor
- Blumeria graminis
Blumeria graminise0pathogen - Gene
- Hv-S/TPKe1
- Outcome
- expressione0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was upregulated after the inoculation of Blumeria graminis to Haynaldia villosa”
e1“Hv-S/TPK gene, a resistance related gene to powdery mildew”
Claim · model-written summary
Screening identified a positive TAC clone containing Hv-S/TPK.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Material
- translocation line 6VS/6ALe0
- Gene
- Hv-S/TPKe0
- Outcome
- a positive TAC was screenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Haynaldia villosa”
e0“Using the specific primers of Hv-S/TPK to screen a genomic TAC (Transformation-competent artificial chromosome) library of translocation line 6VS/6AL, a positive TAC was screened.”
Claim · model-written summary
A 5-kb fragment containing Hv-S/TPK was subcloned and identified.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Gene
- Hv-S/TPKe0
- Outcome
- fragment containing Hv-S/TPK was subcloned and identifiede0
- Value
- 5-kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Haynaldia villosa”
e0“A 5-kb fragment containing Hv-S/TPK was subcloned and identified.”
Claim · model-written summary
The Hv-S/TPK fragment sequence was determined as 5160 bp.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Gene
- Hv-S/TPKe1
- Outcome
- fragment (GenBank Accession No. EU153366) was determinede0
- Value
- 5160-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Haynaldia villosa”
e0“This 5160-bp fragment (GenBank Accession No. EU153366) was determined by specific primer walking.”
e1“A 5-kb fragment containing Hv-S/TPK was subcloned and identified.”
Claim · model-written summary
The Hv-S/TPK genomic sequence contained three introns and four extrons.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Gene
- Hv-S/TPKe0
- Outcome
- three introns and four extronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Haynaldia villosa”
e0“The analysis of Hv-S/TPK genomic sequence showed three introns and four extrons between start code and stop code.”
Claim · model-written summary
The Hv-S/TPK promoter contained W-box and OCS-like resistance-related elements.
- Taxon
- Haynaldia villosaDasypyrum villosumt0
- Gene
- Hv-S/TPKe0
- Outcome
- W-box and OCS-like elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Haynaldia villosa”
e0“In the promoter region of Hv-S/TPK, there were W-box and OCS-like elements which were the elements related to disease resistance.”
Claim · model-written summary
FISH located the Hv-S/TPK TAC clone to a Haynaldia villosa chromosome.
- Taxon
- Haynaldia villosaDasypyrum villosume0
- Gene
- Hv-S/TPKe0
- Outcome
- TAC clone containing Hv-S/TPK was from Haynaldia villosa chromosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The result of fluorescence in situ hybridization (FISH) indicated that the TAC clone containing Hv-S/TPK was from Haynaldia villosa chromosome.”
The same questions in other papers
Blumeria graminis · molecular: expression · Dasypyrum villosumResults agree
1 paper in the same direction as this paper
- positive
inoculation · molecular: expression · Dasypyrum villosumPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.