Abstract
Nitrate reallocation to plant roots occurs frequently under adverse conditions and was recently characterized to be actively regulated by Nitrate Transporter1.8 (NRT1.8) in Arabidopsis (Arabidopsis thaliana) and implicated as a common response to stresses. However, the underlying mechanisms remain largely to be determined. In this study, characterization of NRT1.5, a xylem nitrate-loading transporter, showed that the mRNA level of NRT1.5 is down-regulated by salt, drought, and cadmium treatments. Functional disruption of NRT1.5 enhanced tolerance to salt, drought, and cadmium stresses. Further analyses showed that nitrate, as well as Na(+) and Cd(2+) levels, were significantly increased in nrt1.5 roots. Important genes including Na(+)/H(+) exchanger1, Salt overly sensitive1, Pyrroline-5-carboxylate synthase1, Responsive to desiccation29A, Phytochelatin synthase1, and NRT1.8 in stress response pathways are steadily up-regulated in nrt1.5 mutant plants. Interestingly, altered accumulation of metabolites, including proline and malondialdehyde, was also observed in nrt1.5 plants. These data suggest that NRT1.5 is involved in nitrate allocation to roots and the consequent tolerance to several stresses, in a mechanism probably shared with NRT1.8.
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Claims 10
Claim · model-written summary
Salt, drought, and cadmium down-regulated NRT1.5 mRNA.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- salt, drought, and cadmium treatmentse0
- Gene
- NRT1.5e0
- Outcome
- mRNA level of NRT1.5e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA level of NRT1.5 is down-regulated by salt, drought, and cadmium treatments”
t0“Arabidopsis”
Claim · model-written summary
Functional disruption of NRT1.5 enhanced tolerance to salt, drought, and cadmium.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Functional disruption of NRT1.5e0
- Gene
- NRT1.5e0
- Outcome
- tolerance to salt, drought, and cadmium stressese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional disruption of NRT1.5 enhanced tolerance to salt, drought, and cadmium stresses.”
t0“Arabidopsis”
Claim · model-written summary
Nitrate level increased in nrt1.5 roots.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5e0
- Organ / stage
- rootse0
- Outcome
- nitratee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate, as well as Na(+) and Cd(2+) levels, were significantly increased in nrt1.5 roots”
t0“Arabidopsis”
Claim · model-written summary
Na(+) level increased in nrt1.5 roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate, as well as Na(+) and Cd(2+) levels, were significantly increased in nrt1.5 roots”
t0“Arabidopsis”
Claim · model-written summary
Cd(2+) level increased in nrt1.5 roots.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5e0
- Organ / stage
- rootse0
- Outcome
- Cd(2+) levelse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate, as well as Na(+) and Cd(2+) levels, were significantly increased in nrt1.5 roots”
t0“Arabidopsis”
Claim · model-written summary
Stress-response genes Na(+)/H(+) exchanger1, Salt overly sensitive1, and Pyrroline-5-carboxylate synthase1 were up-regulated in nrt1.5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5 mutant plantse0
- Outcome
- Na(+)/H(+) exchanger1, Salt overly sensitive1, Pyrroline-5-carboxylate synthase1e0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Na(+)/H(+) exchanger1, Salt overly sensitive1, Pyrroline-5-carboxylate synthase1, Responsive to desiccation29A, Phytochelatin synthase1, and NRT1.8 in stress response pathways are steadily up-regulated in nrt1.5 mutant plants.”
t0“Arabidopsis”
Claim · model-written summary
Responsive to desiccation29A, Phytochelatin synthase1, and NRT1.8 were up-regulated in nrt1.5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5 mutant plantse0
- Outcome
- Responsive to desiccation29A, Phytochelatin synthase1, and NRT1.8e0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Responsive to desiccation29A, Phytochelatin synthase1, and NRT1.8 in stress response pathways are steadily up-regulated in nrt1.5 mutant plants.”
t0“Arabidopsis”
Claim · model-written summary
Proline accumulation was altered in nrt1.5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5e0
- Outcome
- prolinee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altered accumulation of metabolites, including proline and malondialdehyde, was also observed in nrt1.5 plants”
t0“Arabidopsis”
Claim · model-written summary
Malondialdehyde accumulation was altered in nrt1.5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nrt1.5e0
- Outcome
- malondialdehydee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altered accumulation of metabolites, including proline and malondialdehyde, was also observed in nrt1.5 plants”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that NRT1.5 participates in root nitrate allocation and stress tolerance through a mechanism probably shared with NRT1.8.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NRT1.5e0
- Outcome
- nitrate allocation to roots and the consequent tolerance to several stressese0
- Qualifier
- probably shared with NRT1.8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NRT1.5 is involved in nitrate allocation to roots and the consequent tolerance to several stresses, in a mechanism probably shared with NRT1.8”
t0“Arabidopsis”
The same questions in other papers
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